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Updated: Feb 22, 2026

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
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A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

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开发一种多重ddPCR试验,用于同时绝对量化细菌,真菌和人类DNA

Guohong Huang1, Torrey L Gallagher1, Gregory J Tsongalis1

  • 1Department of Pathology and Laboratory Medicine, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire, United States of America.

PloS one
|February 20, 2026
PubMed
概括

一种新的多重滴滴数字PCR试验准确量化细菌和真菌DNA,同时测量人类DNA污染. 这种质量控制工具提高了在元基因组学研究中的微生物检测灵敏度.

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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