相关实验视频
Updated: May 6, 2026

05:05
A Modified Precipitation Method to Isolate Urinary Exosomes
Published on: January 16, 2015
18.1K
瘤衍生的细胞外囊泡的淋巴细胞路由证实了尿液活检
Shota Kawaguchi1, Taiga Ajiri1, Rina Mitsuya2
1Department of Life Science and Technology, Institute of Science Tokyo, Nagatsuta 4259, Midori-ku, Yokohama 226-8501, Japan.
Science advances
|February 20, 2026
概括
来自瘤的小细胞外囊泡 (sEVs) 被质细胞活跃地输送到尿液中. 这一发现为尿路癌症诊断和液体活检提供了一种机制.
科学领域:
- 生物医学科学 生物医学科学
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 尿小细胞外囊泡 (sEVs) 显示出非侵入性癌症诊断的潜力.
- 瘤衍生的sEVs到达尿液的机制目前尚不清楚.
研究的目的:
- 阐明由瘤衍生的sEVs被排泄到尿液中的机制.
- 为了研究质细胞在处理循环中的瘤衍生的sEVs中的作用.
主要方法:
- 使用CRISPR指导RNA标记和生物发光/光绿色增强纳米灯 (GeNL) 标记的sEVs来自各种癌症类型 (瘤,肺,胰腺).
- 在多个小鼠模型中,使用体内和体外分析跟踪从瘤到尿液的sEVs.
- 在淋巴细胞处理过程中分析了sEVs以检测体积和表面组成的变化.
主要成果:
- 已证明瘤衍生的sEVs通过球细胞进入尿液的活性转细胞.
- 在淋巴细胞处理后观察到sEV大小和表面组成的变化.
- 与血相比,GeNL标记的sEV在尿液中的度显著更高,表明有选择性排泄.
结论:
- 淋巴细胞活跃地处理并将循环中的瘤衍生的sEVs分泌到尿液中.
- 建立了对尿液中的sEVs的机制性理解,支持开发用于癌症检测的尿液活检.
相关概念视频
Renal Corpuscle
8.5K
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
8.5K
Renal Tubule and Collecting Duct
4.5K
The renal tubule is divided into three parts: the proximal convoluted tubule (PCT), the Loop of Henle (LOH), and the distal convoluted tubule (DCT).
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
4.5K
Ureters
2.2K
The ureters are retroperitoneal tubes located on either side of the vertebral column. They are responsible for transporting urine from each kidney to the urinary bladder. These tubes have thick walls and are approximately 25-30 cm long. Their diameter is around 10 mm at the renal pelvis, gradually narrowing to 1 mm as the ureter obliquely enters the posterior bladder wall through the ureteric orifices. The shape of these orifices is slit-like, which helps to prevent urine backflow toward the...
2.2K
Imaging Studies I: Kidney, Ureter, and Bladder Studies
884
Kidney, Ureter, and Bladder (KUB) StudiesKidney, Ureter, and Bladder (KUB) studies are standard diagnostic imaging procedures used to assess the anatomy of the urinary system. They are commonly utilized for patients experiencing abdominal pain or urinary symptoms. By using a simple X-ray of the abdomen, KUB studies can reveal structural and pathological abnormalities within the kidneys, ureters, and bladder. These studies are particularly valuable in diagnosing kidney stones, urinary...
884
Imaging Studies V: Intravenous Urography and Retrograde Pyelography
3.5K
IntroductionIntravenous Urography (IVU) and Retrograde Pyelography (RP) are important diagnostic imaging techniques used to evaluate the urinary system. These methods help identify structural abnormalities, obstructions, and functional issues in the kidneys, ureters, and bladder. Both procedures use iodine-based contrast media to enhance the visibility of urinary tract structures on X-ray images, though they differ in their methods and indications.1. Intravenous Urography (IVU)Intravenous...
3.5K

