一个高度稳定的C18修饰的聚乙烯-双乙烯静止阶段在多孔上,用于HPLC
Zhipeng Zhu1, Min Zhang1, Xiaohui Yan1
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, State Key Laboratory Base of Novel Functional Materials and Preparation Science, School of Materials Science & Chemical Engineering, Ningbo University, Ningbo, Zhejiang, 315211, China.
这项研究开发了一种新的C18静止相在上,用于高性能液态染色学 (HPLC). 新阶段提供了增强的化学稳定性和改善了芳香化合物和蛋白质的分离.
科学领域:
- 染色体学 染色体学 是一种染色学.
- 材料科学 材料科学 材料科学
- 分析化学 分析化学
背景情况:
- 聚乙烯-双乙烯 (PS/DVB) 静止相在二氧化上对于色谱学至关重要.
- 提高化学稳定性和减少不可逆转的相互作用是关键的挑战.
- 表面修改旨在提高复杂分离的性能.
研究的目的:
- 在多孔上合成和描述化学稳定的PS/DVB静止相.
- 用HPLC评估其在分离芳香化合物和蛋白质方面的性能.
- 为了减轻不可逆转的π-π相互作用,并改善色谱特性.
主要方法:
- 表面启动的原子转移基聚合 (SI-ATRP) 用于PS/DVB接种.
- 通过Friedel-Crafts化引入C4,C8和C18链.
- 使用FTIR,TGA和吸附-溶解的特性.
- 高性能液态色谱 (HPLC) 用于分离分析.
主要成果:
- SiL-PSD-C18相表现出极好的化学稳定性,耐0.1M NaOH超过48小时.
- 对芳香化合物而言,显著减少峰值尾部和改善峰值对称性.
- 减少移动相位平衡时间和高保留时间稳定性用于蛋白质分离 (RSD < 0.34%).
- 在加速老化下,在16000个柱体体积后保持了>98%的柱子效率.
- 在测试的柱子中表现出最低的柱子出血.
结论:
- 开发的SiL-PSD-C18静止相提供了卓越的化学稳定性和色谱性能.
- 它有效地解决了分离芳香化合物和蛋白质的现有相的局限性.
- 这种材料代表了对要求高的HPLC应用的有希望的进步.
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