CuO纳米颗粒触发了与质亡相关的线粒体损伤和斑马鱼肠道微生物群-代谢乱
Luyao Zhang1, Danyang Zhang1, Silu Li2
1School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
氧化铜纳米颗粒 (CuO NPs) 通过破坏线粒体和破坏肠道微生物,比离子铜引起更大的肠道毒性. 这项研究揭示了形式依赖性毒性和肠道微生物群在CuO NP健康风险中的作用.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 纳米技术纳米技术
背景情况:
- 氧化铜纳米颗粒 (CuO NPs) 被广泛使用,增加了环境暴露风险.
- 与离子铜相比,对CuO NPs的慢性毒性了解较少.
- 长期暴露于铜的肠道毒性需要进一步调查.
研究的目的:
- 评估斑马鱼中CuO NPs与硫酸铜 (CuSO4) 的慢性肠道毒性.
- 阐明形成依赖的铜毒性背后的机制.
- 评估对肠道微生物群和宿主新陈代谢的影响.
主要方法:
- 斑马鱼暴露于CuO NP或CuSO4.4的环境相关度.
- 综合策略:超结构分析,生物化学分析,肠道微生物群分析,代谢学.
- 分析线粒体功能,代谢途径和微生物社区结构.
主要成果:
- 无论是CuO NPs还是CuSO4,都诱导了线粒体损伤,代谢干扰和肠道失调.
- 与CuSO4相比,CuO NP的肠道毒性明显高于CuSO4.
- CuO NPs直接损害了线粒体,激活了cuproptosis,并改变了氨基酸,脂质和核酸代谢.
- CuO NPs对肠道微生物群与宿主代谢物相互作用的影响更大.
结论:
- 铜形式决定了肠道毒性的程度.
- 由于直接的线粒体损伤和强大的cuproptosis通路激活,CuO NPs带来了更大的慢性健康风险.
- 微生物群-代谢物交叉对CuO NP诱导的不良影响至关重要,为风险评估提供了信息.
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