SIGLEC-1表达单细胞作为诊断生物标记在儿科I型干扰子介导疾病
Valentina Matteo1, Hana Zeric2, Elena Loricchio3
1Laboratory of Immuno-Rheumatology, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy; Department of Biomedicine and Prevention, PhD in Immunology, Molecular Medicine and Applied Biotechnology, University of Rome "Tor Vergata", Rome, Italy.
The Journal of allergy and clinical immunology
|February 20, 2026
概括
单细胞上的SIGLEC-1表达是I型干扰素 (IFN) 激活的可靠生物标志物,为诊断IFN介导疾病提供了I型干扰素评分 (IS) 的经济有效替代方案.
科学领域:
- 免疫学 免疫学 免疫学
- 分子诊断学 分子诊断学
- 生物标志物发现发现
背景情况:
- I型干扰素 (IFN) 介导的疾病由于各种症状和现有测试的局限性,如I型干扰素 (IFN) 评分 (IS) 等,造成了诊断上的挑战.
- 需要一个实用和可靠的生物标志物来准确诊断和监测这些疾病.
研究的目的:
- 评估SIGLEC-1在单细胞上的表达作为检测I型IFN激活的可行的生物标志物.
- 评估SIGLEC-1的诊断准确性和分析稳定性,作为I型IS的替代品.
主要方法:
- 一项综合后期和前性研究,涉及109名疑似IFN介导疾病的患者.
- 使用流细胞计 (MFI和% SIGLEC-1+单细胞) 量化SIGLEC-1表达.
- 分析验证包括全血与PBMC的比较,不同的细胞计和折叠变化正常化的评估.
主要成果:
- 在回顾性队列中,SIGLEC-1表达与I型IS有很强的相关性 (R2=0.76) 并显示出出色的诊断准确性 (AUC 0.99-0.98).
- 预期队列中的优化切断线完全区分干扰和其他诊断 (AUC = 1.0) 之间的干扰.
- 分析验证证实了不同样本类型和仪器的稳定性,折叠变换正常化将平台变化最小化.
结论:
- 流量细胞计SIGLEC-1是I型IS的强大,经济高效和可复制的替代品.
- SIGLEC-1支持I型IFN介导疾病的诊断,患者分层和纵向监测.
- 实施SIGLEC-1测试可以改善干扰性疾病患者的管理.
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