罗盘子单元Bre2调节染色体重塑剂Arp9,以控制Aspergillus flavus的亚毒素合成和毒性
Zhenhong Zhuang1, Minghui Sun2, Dandan Wu1
1State Key Laboratory of Agricultural and Forestry Biosecurity, Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, Proteomic Research Center, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
综合体子单元Bre2调节了阿斯伯吉卢斯黄菌 (Aspergillus flavus) 中的阿弗拉托克辛B1 (AFB1) 生产和真菌病原性. 这涉及通过染色体重塑剂Arp9进行表观遗传控制,提供了对抗真菌毒素的新策略.
科学领域:
- 菌类学 菌类学是指菌类学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 阿斯伯吉勒斯黄 (Aspergillus flavus) 产生阿弗拉托克辛B1 (AFB1),这是一种对人类健康的严重危害.
- COMPASS (与Set1相关的蛋白质综合体) 综合体调节AFB1生物合成和A. flavus病毒性,但其机制尚未完全理解.
研究的目的:
- 阐明COMPASS子单元Bre2调节AFB1生物合成,真菌形态发生和毒性的机制.
- 确定涉及Bre2-介导表观遗传调节的下游目标和途径.
主要方法:
- 染色体免疫沉测序 (ChIP-seq) 用于识别Bre2标.
- 生物化学分析以研究蛋白质相互作用.
- 使用测序 (ATAC-seq) 测定转化酶可访问的染色质,以评估染色质的可访问性.
主要成果:
- Bre2调节H3K4甲基化,影响AFB1的生物合成,形态发生和毒性.
- 染色体重塑因子 (CRF) Arp9是Bre2的直接目标,并与其他CRF (RSC8,Arp7,Sth1) 相互作用.
- Arp9通过改变毒性相关基因的染色质构造来影响真菌的致病性.
结论:
- 涉及Bre2和Arp9的表观遗传信号通路调节了关键的真菌过程.
- Arp9在调节色素结构中的作用为控制病原菌和真菌毒素提供了潜在的点.
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