用于食品应用的亚临界水解:肉蛋白的温度依赖转化和生物活性
Jong Won Lee1, SangYoon Lee1, Geun-Pyo Hong2
1Department of Food Science & Biotechnology, and Carbohydrate Bioproduct Research Center, Sejong University, Seoul, South Korea.
NPJ science of food
|February 20, 2026
概括
亚临界水 (SW) 水解有效地将猪肉蛋白转化为生物活性成分. 在250°C的最佳条件下,可以获得高回收率,强大的生物活性和理想的风味,超过了酶性水解 (EH).
科学领域:
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
- 蛋白质化学 蛋白质化学
背景情况:
- 肉蛋白加工通常涉及酶性水解 (EH).
- 探索替代性,高效的肉蛋白价值化方法至关重要.
- 亚临界水 (SW) 水解提供了一种潜在的更环保的方法.
研究的目的:
- 为了评估亚临界水 (SW) 水解用于将猪腰蛋白转化为生物活性成分.
- 为了比较SW水解与酶化水解 (EH) 蛋白质回收和生物活性.
- 为了确定生产高价值肉蛋白成分的最佳SW条件.
主要方法:
- 猪腰经过在100°C至300°C的温度下进行亚临界水 (SW) 水解.
- 化物被分析为蛋白质回收,自由氨基群含量,生物活性 (抗氧化, ACE 抑制),细胞毒性和感官属性 (苦味, umami).
- 结果与通过酶性水解 (EH) 获得的结果进行了比较.
主要成果:
- 与EH相比,在200°C以上的SW处理显著提高了蛋白质回收和自由氨基群含量.
- 在300°C时通过SW实现了最高的蛋白质回收.
- 250°C以上的SW的化物显示出强烈的抗氧化和ACE抑制活性,没有细胞毒性.
- 然而,超过250°C的SW导致了苦味的增加,并抑制了益生菌的生长.
- 与EH相比,在250°C以下的SW治疗导致了痛苦的减少和增强的乌玛米强度.
结论:
- 亚临界水 (SW) 水解是肉蛋白转化的一个有前途的方法.
- 在250°C的最佳SW处理平衡了高蛋白回收,强大的生物活性和有利的味道.
- 这种方法提供了一种可行的策略,用于将猪腰蛋白提升为高价值的食品原料.
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