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来自Lactobacillus helveticus的纳米颗粒上的表面层相关蛋白增强RAW264.7细胞对LPS的反应

Chika Yamamoto¹, Nao Fujiwara¹, Ika Adhani Sholihah²

¹Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido 1-1, Gifu, 501-1193, Japan.

Current microbiology

|February 21, 2026

概括

乳酸细菌是一种乳酸细菌.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
胃肠病学胃肠病学

背景情况:

肠道中的乳酸细菌会影响营养代谢和免疫反应.
益生菌对健康有好处,但与宿主细胞的相互作用机制尚不清楚.
细胞外囊泡 (EV) 和表面层相关蛋白质 (SLAP) 是已知的细胞功能的调节者.

研究的目的:

调查SLAP在Lactobacillus helveticus细胞外囊状纳米颗粒 (EV-LNP) 中的作用.
确定L. helveticusEV-LNP和SLAP如何调节宿主细胞中的免疫反应.

主要方法:

在L. helveticus EV-LNP中识别SLAP.
在SLAP/EV-LNP添加后,在RAW264.7细胞中评估免疫反应 (氧化,IL-6).
调查EV-LNP内部化和NF-κB信号的研究.
分析可诱导的氧化合成酶促进剂活性与SLAP过度表达/删除.
评估EV-LNP/SLAP对大肠杆菌RAW264.7细胞吸收的影响.

主要成果:

在L. helveticus EV-LNPs.中发现了SLAP.
作为EV-LNP或纯化蛋白质的SLAP,在RAW264.7细胞中增强了氧化和IL-6的产生.
L. helveticus的EV-LNP被快速内化,并增强了NF-κB信号传递.
过度表达SLAP增加了可诱导的氧化合成酶促进活性,而删除则取消了这一点.
EV-LNP和SLAP改善了RAW264.7细胞对大肠杆菌的吸收.

结论:

L. helveticus的EV-LNP可以通过SLAP介导的宿主细胞通信激活来增强免疫反应.
SLAP在调节免疫细胞活动和细菌相互作用方面发挥着关键作用.
EV-LNP代表了益生菌与宿主免疫系统相互作用的重要机制.

关键词:

细胞外囊泡中的细胞外囊泡.免疫反应是免疫反应.乳酸细菌是一种乳酸细菌.清除大肠杆菌 (E. coli) 的作用在S层中,与S层相关的蛋白质.

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