相关实验视频

Updated: Feb 23, 2026

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

结合异位基因特异性蛋白质组的多体造揭示了一个具有更高mRNA翻译潜力的EIF4H tranSNP异位基因

Meriem Hadjer Hamadou¹, Laura Alunno¹, Daniele Peroni²

¹Laboratory of Transcriptional Networks; Department of Cellular, Computational, and Integrative Biology, CIBIO, University of Trento, via Sommarive 9, 38123, Trento, Italy.

Molecular & cellular proteomics : MCP

|February 21, 2026

概括

这项研究使用蛋白质组学识别了影响等位基因特异性mRNA翻译的遗传变异. 研究人员验证了EIF4H中的特定变异,展示了一种研究基因表达差异的新方法.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
蛋白质组学是指蛋白质组学.

背景情况:

对等位基因特异的mRNA翻译是基因表达调节的一个层.
研究这种现象可以揭示疾病现象型的个体间差异.

研究的目的:

识别影响等位基因特异性mRNA转换的遗传变异.
开发和验证一种蛋白质组学工作流程,用于研究基因特异性转换在内源性蛋白质水平.

主要方法:

来自HCT116细胞的总和多基因组关联RNA的RNA测序 (RNA-seq),以确定等位异位失衡.
一个蛋白质组学工作流程,将无标签的猎枪分析,分化和并行反应监测 (PRM) 与同位素标记的标准进行验证.
双光核糖体阻滞试验以确认翻译潜力.

主要成果:

提名了52种与异位基因特异转换相关的编码变体,其中16种是非同义的.
对EIF4H R183H误解单核酸变体 (SNV) 的概念验证验证.
变异型H183比参考型R183更为丰富,变异型等位基因显示出增强的翻译潜力.

结论:

这项研究证明了使用异构合的编码变异体的等位基特定蛋白质组的可行性.
开发的方法扩展了研究异位基因特异性mRNA转换的工具.
这项工作突出了基因表达调节的未经探索的层面,对疾病表型有潜在的影响.

关键词:

在EIF4H的基础上,国家统一计划 (SNP) 是一个国家统一计划.亚基因特异性蛋白质组学基失衡是一种基失衡.在mRNA的翻译过程中,mRNA的翻译

更多相关视频

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Published on: April 8, 2018

相关概念视频

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...