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Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Updated: Jul 15, 2026

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

高通量化学蛋白质组学工作流程,用于分析蛋白质化动态.

Rebecca Meelker González¹, Sophia Laposchan¹, Erik Riedel¹

¹Young Investigator Group: Mass Spectrometry in Systems Neurosciences, School of Life Sciences, Technical University of Munich, Freising, Germany.

Nature communications

|February 21, 2026

概括

研究人员开发了一种新的化学蛋白质组学方法来检测含量低的素,这对于了解自身免疫性疾病至关重要. 这种技术显著提高了复杂生物样本中素化位点的识别.

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Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

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Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

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Last Updated: Jul 15, 2026

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

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Published on: March 24, 2012

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Published on: November 17, 2019

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

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Published on: June 21, 2021

科学领域:

生物化学生物化学
蛋白质组学是指蛋白质组学.
化学生物学化学生物学

背景情况:

细胞化是一种关键的翻译后修饰,涉及自身免疫和炎症性疾病.
对于低丰度的素酸的有限检测方法阻碍了全蛋白质组研究.

研究的目的:

开发一个强大的化学蛋白质学工作流程,以加强素的检测和分析.
为了能够全面地绘制素化位点的全蛋白质组范围.

主要方法:

基于glyoxal的衍生与可切割生物素链接器相结合,以实现高效的缩.
质谱测量用于识别富含素酸的物质.
适用于人类初级中性粒细胞和使用Candida albicans的刺激.

主要成果:

在0.1%以下的丰度下,检测素的数量增加了>10倍.
监测中性粒细胞中素化动态调节,包括由GSK484.4进行剂量依赖抑制.
在不同的刺激中确定了一种保存的"核心素瘤",并观察到结构蛋白的广泛素化.

结论:

开发的工作流显著提高了研究素化反应的灵敏度和吞吐量.
这种方法有助于研究素在各种生物过程和疾病中的作用.
能够为未来免疫学和细胞生物学领域的研究,详细地绘制素化部位的地图.