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Updated: Jul 15, 2026

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

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高通量化学蛋白质组学工作流程,用于分析蛋白质化动态

Rebecca Meelker González1, Sophia Laposchan1, Erik Riedel1

  • 1Young Investigator Group: Mass Spectrometry in Systems Neurosciences, School of Life Sciences, Technical University of Munich, Freising, Germany.

Nature communications
|February 21, 2026
PubMed
概括

研究人员开发了一种新的化学蛋白质组学方法来检测含量低的素,这对于了解自身免疫性疾病至关重要. 这种技术显著提高了复杂生物样本中素化位点的识别.

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Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

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Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation07:16

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相关概念视频

Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.