在肺动脉高血压中的生物标志物的基于生物信息学的识别和实验验证
Ruohan Jia1, Ke Wang1, Yize Liu1
1Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.
肺动脉高血压 (PAH) 诊断需要改进. 研究人员确定了五个关键基因 (NOP58,DDX21,ABCE1,CDC5L,HSP90AA1) 作为潜在的早期诊断生物标志物和PAH的治疗标.
科学领域:
- 基因组学和生物信息学
- 心血管研究研究心血管研究
- 免疫学 免疫学 免疫学
背景情况:
- 目前的肺动脉高血压 (PAH) 诊断依赖于侵入性手术,限制了早期检测.
- 现有的成像方法缺乏灵敏度,并且是可变的,往往导致晚期诊断.
- 了解PAH的发病过程和开发新的诊断和治疗策略至关重要.
研究的目的:
- 确定肺动脉高血压 (PAH) 的新型诊断生物标志物和治疗点.
- 研究与PAH相关的分子机制和免疫场景.
- 探索PAH潜在的基因导向个性化治疗方法.
主要方法:
- 不同基因表达分析,基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 对GSE113439数据集的丰富分析.
- 权重基因共同表达网络和蛋白质-蛋白质相互作用网络分析以确定枢纽基因.
- 建立一个PAH大鼠模型,验证细胞和组织中的枢纽基因表达,以及免疫透分析.
主要成果:
- 在PAH中发现了547个不同表达的基因.
- 与PAH相关的关键途径包括焦点粘附,血管平滑肌肉收缩,RNA降解,铁,和2-氧碳酸代谢.
- NOP58,DDX21,ABCE1,CDC5L和HSP90AA1在PAH患者中显著上调,并与免疫细胞差异有关,特别是中性粒细胞和巨细胞.
结论:
- NOP58,DDX21,ABCE1,CDC5L和HSP90AA1显示出作为PAH的新型诊断生物标志物具有前途.
- 这些基因代表PAH治疗的潜在治疗标.
- 需要在大型研究中进一步验证,以确认临床适用性和探索个性化疗法.
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