MALDI-TOF对Sanguibacteroides justesenii的错误鉴定:通过16S rRNA测序得到确认
Valentin Bedfert1, Stéphane Corvec2, Marion Leterrier-Plong1
1Laboratoire de Biologie Médicale, GCS Biologie85, Centre Hospitalier Départemental de Vendée, 85000 La Roche sur Yon, France.
Diagnostic microbiology and infectious disease
|February 22, 2026
概括
罕见的无氧细菌Sanguibacteroides justesenii在一个感染性休克病例中被MALDI-TOF MS错误识别. 16S rRNA基因测序证实了S.justesenii,强调了当MALDI-TOF结果对罕见物种不确定时,需要使用分子方法.
科学领域:
- 医学微生物学 医学微生物学
- 临床诊断 临床诊断 临床诊断
- 传染性疾病 传染性疾病
背景情况:
- 桑吉巴克特罗伊德 (Sanguibacteroides justesenii) 是一种罕见的,具有不明确临床意义的,具有克拉姆阴性无氧性细菌.
- 通过传统的诊断方法,准确识别S.justesenii是一个挑战.
研究的目的:
- 报告一例S.justesenii细菌性病例,该病例发生在患有结肠穿孔后败血症休克的患者身上.
- 突出MALDI-TOF MS在识别罕见无氧细菌方面的局限性,并强调分子确认方法的重要性.
主要方法:
- 一个58岁的女性患者的病例报告,患有急性心力衰竭和随后的败血症休克.
- 使用MALDI-TOF MS (Bruker®) 和16S rRNA基因测序进行血液培养的分析.
- 在MALDI-TOF MS和16S rRNA基因测序之间对鉴定结果的比较.
主要成果:
- MALDI-TOF MS最初错误地将这种分离物识别为Gabonibacter massiliensis (得分为2.19).
- 16S rRNA基因测序证实该分离物是S. justesenii.
- 在两个实验室中重复进行的MALDI-TOF MS分析显示出相同的错误识别,表明稀有物种的数据库限制.
结论:
- 这一案例强调了识别S.justesenii的挑战,以及由于数据库限制,MALDI-TOF MS可能会错误识别.
- 像16S rRNA基因测序这样的分子方法对于精确识别罕见无氧细菌至关重要,因为MALDI-TOF MS的结果是可疑的.
- 患者在接受了阿莫西西林和甲尼达治疗后成功康复,这一案例强调了在诊断罕见的血液感染时需要分子确认的必要性.
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