Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

9.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.8K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

2.1K
2.1K
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.8K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

4.5K
4.5K
Proteomics01:33

Proteomics

10.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Beyond AI-Driven Curve Fitting: Mechanistic Insight as the Cornerstone of Reliable Sensing and Accurate Fitting.

Small methods·2026
Same author

Seven Models for Outer Surface-Only Functionalized Nanofluidic Systems.

ACS nano·2026
Same author

Site-specific post-translational modification detection by polar charged engineered MspA nanopores.

Chemical science·2026
Same author

Dual-Interface Nanopore Array Sensor Revealing the Synergistic Oscillations of Lactate Efflux in Cancer Cells.

Angewandte Chemie (International ed. in English)·2026
Same author

A robust Au-C[triple bond, length as m-dash]C anchoring group greatly improves the signal stability of electrochemical aptamer-based sensors for <i>in vivo</i> measurements.

Chemical science·2026
Same author

Photoactivated Signaling Networks using DNA-Based Synthetic Organelles as Biomimetic Protocells.

Angewandte Chemie (International ed. in English)·2026

相关实验视频

Updated: Feb 24, 2026

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
08:12

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Published on: July 11, 2017

7.8K

一个共价向平台用于蛋白质动态跟踪.

Bochao Chen1,2, Yong Cheng1, Zairong Men1

  • 1State Key Laboratory of Geomicrobiology and Environmental Changes, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, China.

Analytical chemistry
|February 23, 2026
PubMed
概括

研究人员开发了一种新的共价向成像 (CTI) 平台,用于在活细胞中长期跟踪蛋白质. 这一突破使得蛋白质动态的稳定监测成为可能,有助于疾病机制研究和治疗开发.

更多相关视频

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

1.2K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

5.5K

相关实验视频

Last Updated: Feb 24, 2026

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
08:12

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Published on: July 11, 2017

7.8K
Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

1.2K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

5.5K

科学领域:

  • 生物医学成像学 生物医学成像学
  • 细胞动态 细胞动态
  • 分子生物学分子生物学

背景情况:

  • 蛋白质动态的长期成像对于了解疾病机制和开发诊断/治疗方法至关重要.
  • 目前的成像探测器具有局限性,包括短时间的细胞内保留和快速清除,阻碍了有效的长期研究.
  • 需要先进的成像工具,允许对蛋白质行为进行持续的细胞内观测.

研究的目的:

  • 开发一种新型的共价向成像 (CTI) 平台,用于在活细胞内进行强大和长期的蛋白质动态跟踪.
  • 克服当前有关细胞内保留和清除的探针的局限性.
  • 证明该平台在生物医学应用中的有效性和通用性.

主要方法:

  • 开发一种结合的探针,其中包含一个maleimide组,用于与标蛋白形成共价键.
  • 利用共价键形成来限制光分子转子,从而激活稳定,长期的光信号.
  • 使用特定探针 (MXQ) 验证概念验证,用于追踪MCF-7细胞中的X结合的亡蛋白抑制剂 (XIAP).

主要成果:

  • 该CTI平台成功实现了XIAP蛋白动态在活体MCF-7细胞中的长期,稳定的跟踪.
  • 共价连接确保了长时间的探头保留和信号稳定性,克服了以前方法的局限性.
  • 该探测器可以精确监测细胞内XIAP水平对治疗效应的情况.

结论:

  • 开发的共价向成像 (CTI) 平台为生物细胞中长期蛋白质动态跟踪提供了可泛化和有效的解决方案.
  • 这种方法克服了现有的成像探针的关键局限性,为加强疾病机制研究铺平了道路.
  • 该CTI平台具有显著的潜力,可以加速跨多种疾病领域的生物医学应用.