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Updated: Feb 24, 2026

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

在瘤组织和食品样本中通过衍生辅助的MALDI-TIMS-MS进行iralα-Hydroxy酸歧视

Simin Zhang¹, Thomas K Y Lam¹, Jianing Wang¹

¹State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong SAR 999077, P. R. China.

Journal of the American Society for Mass Spectrometry

|February 23, 2026

概括

这项研究引入了一种新的方法,用于区分像2-二 (2-HG) 和乳酸 (LA) 这样的性代谢物,使用性衍生和质谱学. 该技术准确地确定了反体比率,这对于代谢障碍研究和诊断至关重要.

科学领域:

分析化学分析化学
代谢学代谢学代谢学
生物化学生物化学

背景情况:

基质代谢物分析对于了解代谢障碍和疾病生物标志物至关重要.
准确的反体比率测定对于临床诊断和食品安全至关重要.

研究的目的:

开发和优化一种方法来进行α-基酸的奇拉区分,特别是2-基酸 (2-HG) 和乳酸 (LA).
为了能够精确地确定复杂的生物和食品矩阵中的反体比率.

主要方法:

整合了一种性衍生策略与矩阵辅助激光脱/电离 (MALDI) 和被困离子移动性谱学 (TIMS).
优化衍生试剂 (TSPC和DATAN) 和金属离子添加物用于二聚体转化.
高分辨率的反体分离和量化.

主要成果:

用高分辨率成功地分离了2-HG和LA等离子体.
已证明能够确定低至5.8%的反体比率.
在IDH突变瘤组织中敏感识别2-HG,精确确定发酵食品中的LA反体.

结论:

开发的方法提供了一个强大的工具,用于复杂样品中性代谢物分析.
这种方法在推进生物标志物查,食品安全,制药开发和临床诊断方面具有重大潜力.

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