将多重代谢工程与适应性进化策略相结合,用于在Yarrowia lipolytica中高水平的酸生产
Tao Sun1, Mei-Li Sun1, Lu Lin1
1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, People's Republic of China.
Synthetic and systems biotechnology
|February 23, 2026
概括
工程酵母Yarrowia lipolytica有效地从糖醇和葡萄糖中产生酸. 这项研究通过代谢工程和适应性进化来增强酸生物合成,以实现可持续的生物制造.
科学领域:
- 生物技术和合成生物学
- 代谢工程是代谢工程.
- 工业微生物学 工业微生物学
背景情况:
- 酸是一种重要的平台化学物质,具有多种工业应用.
- 酸的传统合成方法是能源密集型和环境问题.
- 生物技术生产提供了一个可持续的替代方案.
研究的目的:
- 通过使用先进的代谢工程和适应性进化技术,增强Yarrowia lipolytica中的酸生物合成.
- 为了优化从可再生原料如甘油和葡萄糖中生产酸的生产.
- 为了深入了解影响酸生产的代谢途径.
主要方法:
- 多重代谢工程策略应用于Yarrowia lipolytica.
- 适应性实验室进化被用来提高菌株性能和基质利用率.
- 关键的酶,包括糖酸脱酶 (Sdh),被针对减弱.
- 运输器工程是为了增强葡萄糖吸收而进行的.
- 转录组分析和反向代谢工程被用来阐明途径.
主要成果:
- 工程菌株从糖中获得高酸标位 (130.99 g/L),具有显著的产量 (0.35 g/g) 和生产力 (0.70 g/L·h)).
- 一种进化的菌株从葡萄糖 (106.68 g/L) 中表现出高效的酸生产,产量为0.32 g/g,生产率为0.64 g/L·h,克服了增长滞后阶段.
- 鉴定出4-hydroxyphenylpyruvate dioxygenase (4-Hppd) 酶对于恢复葡萄糖上Sdh缺乏菌株的生长至关重要.
结论:
- 代谢工程和适应性进化是增强酸生产的有效策略. 脂质过敏症 (lipolytica) 是一种
- 这些工程菌株表明,从各种原料中实现可持续和高效的酸生物制造具有很大的潜力.
- 了解关键的代谢调节者,如4-Hppd,为进一步改善菌株提供了新的途径.
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