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Crystal Growth: Principles of Crystallization

Crystal Growth: Principles of Crystallization

Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...

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Updated: Feb 24, 2026

Protein Crystallization for X-ray Crystallography

Protein Crystallization for X-ray Crystallography

Published on: January 16, 2011

在拥挤的酶溶液中形成晶体相.

Ivaylo L Dimitrov^1,2

¹Institute of Physical Chemistry "Rostislaw Kaischew", Bulgarian Academy of Sciences Acad. G. Bonchev Str., bl. 11 1113 Sofia Bulgaria idimitrov@ipc.bas.bg.

|February 23, 2026

概括

溶液中的lyszyme拥挤可以通过改变蛋白质-盐相图来触发快速晶体形成. 这种受控的方法有助于预测结晶核化时间.

科学领域:

生物化学生物化学
晶体学晶体学是指结晶学.
材料科学材料科学材料科学

背景情况:

蛋白质结晶对于结构生物学和药物开发至关重要.
了解拥挤环境中的结晶是模仿细胞条件的关键.

研究的目的:

为了在拥挤的环境中研究酶结晶.
探索受控拥挤对晶相形成和核形成的影响.

主要方法:

使用的微升容量滴是缓冲式酶溶液 (pH 4.0) 与变化的初始蒸汽压力.
在没有添加沉剂的情况下,达到高的lyszyme度 (超过250 mg ml-1) 和缓冲盐度 (0.31 M酸).
在18-33°C的温度范围内监测结晶动态.

主要成果:

逐渐的酶拥挤导致了突然的晶体阶段形成.
转移稳定的区域实际上扩大了,核化区域向不稳定的区域缩小了.
核化事件发生在合作中,随后是快速的晶体生长占据了大部分的滴量.
观察到晶体形成的最低温度依赖性,主要影响平衡速率.

结论:

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Last Updated: Feb 24, 2026

Protein Crystallization for X-ray Crystallography

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Published on: January 16, 2011

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From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

控制的蛋白质拥挤是诱导和管理结晶的有效策略.
开发的方法允许预测晶体核形成时间.
这种方法为生物分子凝聚和相分离现象提供了洞察力.