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相关概念视频

The Z-Scheme of Electron Transport in Photosynthesis01:34

The Z-Scheme of Electron Transport in Photosynthesis

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The light reactions of photosynthesis assume a linear flow of electrons from water to NADP+. During this process, light energy drives the splitting of water molecules to produce oxygen. However, oxidation of water molecules is a thermodynamically unfavorable reaction and requires a strong oxidizing agent. This is accomplished by the first product of light reactions: oxidized P680 (or P680+), the most powerful oxidizing agent known in biology. The oxidized P680 that acquires an electron from the...
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Electron Transport Chains01:28

Electron Transport Chains

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The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
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Photosystem I01:27

Photosystem I

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Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
70.4K
Electron Transport Chain Components01:29

Electron Transport Chain Components

1.1K
The electron transport chain (ETC) is a crucial metabolic pathway that facilitates energy conversion in prokaryotic and eukaryotic cells. In eukaryotes, the ETC comprises four membrane-associated protein complexes in the inner mitochondrial membrane. In prokaryotes, the ETC in the plasma membrane can vary in composition, with fewer or different complexes depending on the organism and environmental conditions. These complexes transfer electrons from electron donors, such as NADH and FADH2, to...
1.1K
Role of Reduced Coenzymes NADH and FADH₂01:29

Role of Reduced Coenzymes NADH and FADH₂

17.5K
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
17.5K
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

9.4K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
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Mechanistic implications of excited high-spin states, spin-spin coupling, and differential [2Fe-2S]<sup>+</sup> cluster temperature relaxations in the electron-bifurcating NfnABC from <i>Thermococcus sibiricus</i>.

Dalton transactions (Cambridge, England : 2003)·2026
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A photosynthetic-respiratory electron transport chain chimera based on photosystem I and cytochrome <i>c</i> oxidase on graphene oxide.

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相关实验视频

Updated: Feb 24, 2026

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5&#8242;-Phosphate
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Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

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照片诱导的电子转移在基于弗拉文的电子双分支中告知了路径合.

Seth A Wiley1, Carolyn E Lubner1

  • 1Biosciences Center, National Laboratory of the Rockies, Golden, Colorado 80401, United States.

ACS bio & med chem Au
|February 23, 2026
PubMed
概括

基于黄素的电子分叉 (FBEB) 使用Nfn酶来产生高能电子. 新的低温EPR方法揭示了这种酶如何控制电子流,为能量转化提供了洞察力.

科学领域:

  • 生物化学 生物化学
  • 酶学 是一种酶学.
  • 生物能源学 生物能源学

背景情况:

  • 基于黄素的电子分叉 (FBEB) 是能量转换的关键酶过程.
  • 在 *Pyrococcus furiosus* 中的依赖NADH的铁素:NADP+-氧化还原酶 (Nfn) 使用FBEB来驱动不良反应.
  • 了解NFN控制高能电子的机制对于生物能源学研究至关重要.

研究的目的:

  • 调查Nfn.fn在低电位通路中的电子控制机制.
  • 阐明蛋白质环境在管理短寿命,高能电子中间体中的作用.
  • 了解Nfn.内的电子转移步骤和辅因子相互作用.

主要方法:

  • 低温光刺激的适应与电子磁共振 (EPR) 谱学相结合.
  • 在冷温度下积累和表征短寿命的基质中间体.
  • 在电子分支过程中分析辅因子相互作用,包括 [4Fe-4S] 集群.

主要成果:

  • 在冷却温度下对NADPH照明时观察到激素中间体和附近的 [4Fe-4S] 集群的巧合生长.
  • 光生成的偏磁物种在液温度下稳定,在变暖时重组.
  • 确定了一种涉及关键残留物运动的潜在封闭机制,影响电子流的可逆性.
关键词:
电子二分支的电子二分支.酶 是一种酶.机理机制 机理机制摄影化学的使用.蛋白质 蛋白质 蛋白质反应中间体 反应中间体氧化还原反应 氧化还原反应

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Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
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Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

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Last Updated: Feb 24, 2026

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Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
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Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

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Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
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Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

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结论:

  • 这项研究为Nfn低电位通路中的电子转移动态和辅因子相互作用提供了新的见解.
  • 低温EPR成功地探测不稳定的中间体,增强了对FBEB的机制理解.
  • 一个基于残留的封闭机制可以调节Nfn.中的电子流的方向性.