Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The mitochondrial logic of inflammaging: how energy imbalance drives fibroblast SASP and tissue-specific aging.

Frontiers in immunology·2026
Same author

Effect of evidence-based predictive nursing on postoperative infection and recovery outcomes in cesarean delivery: A case-control study.

Medicine·2026
Same author

Comprehensive genomic and computational insights into Brucella suis: pan-genome analysis, evolutionary perspectives, and in-silico vaccine design.

BMC genomics·2026
Same author

Dynamic visualization of physiological CaMKII activity using sensitive FRET biosensors.

bioRxiv : the preprint server for biology·2026
Same author

A highly sensitive genetically encoded red cAMP sensor for multiplex imaging in vivo.

Nature communications·2026
Same author

Genetically targeted mTORC1 inhibitor reveals transcriptional control by nuclear mTORC1.

Nature chemical biology·2026

相关实验视频

Updated: May 31, 2026

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
10:59

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

10.7K

基于FRET的高度敏感的化学遗传激酶生物传感器.

Xinchang He, Daojia R Zhou, Yu Huan

    bioRxiv : the preprint server for biology
    |February 23, 2026
    PubMed
    概括

    化学遗传生物传感器克服了传统FRET传感器的局限性,为研究细胞信号提供了高灵敏度和动态范围. 这些先进的工具使复杂的生物系统中生物化学活动的精确,活细胞成像成为可能.

    科学领域:

    • 细胞生物学 细胞生物学
    • 生物化学 生物化学
    • 分子成像学分子成像学

    背景情况:

    • 光蛋白生物传感器可以在活细胞中测量生化活动.
    • 基于Förster共振能量转移 (FRET) 的生物传感器在动态范围和光谱特性方面存在限制.
    • 化学遗传学方法通过将合成光体与自我标记蛋白质标签相结合来提供解决方案.

    研究的目的:

    • 开发高度灵敏的,基于FRET的化学遗传生物传感器,具有红移排放和改进的动态范围.
    • 为了证明这个平台对各种生物分子的多功能性,包括激酶,GTPase和第二信使.
    • 为了使细胞和组织中的信号网络能够进行强大的多重成像和可视化.

    主要方法:

    • 配对光蛋白捐赠体与与远红色光体结合的HaloTag受体.
    • 开发和验证用于多个酶标,小GTPases和第二信使的化学遗传生物传感器.
    • 使用双光子光终身成像用于在急性脑部切片中可视化.

    主要成果:

    • 创建了一套高度敏感的化学遗传FRET生物传感器,具有红移发射和前所未有的动态范围.
    • 该平台在各种目标中展示了可通用性,同时保持了高灵敏度.
    • 实现了信号网络的强大的多重活动成像和脑切片中酶活动的清晰可视化.

    更多相关视频

    Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
    11:23

    Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

    Published on: June 30, 2019

    6.7K
    Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
    09:39

    Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

    Published on: March 31, 2022

    3.7K

    相关实验视频

    Last Updated: May 31, 2026

    Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
    10:59

    Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

    Published on: February 10, 2014

    10.7K
    Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
    11:23

    Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

    Published on: June 30, 2019

    6.7K
    Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
    09:39

    Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

    Published on: March 31, 2022

    3.7K

    结论:

    • 化学遗传生物传感器为研究具有增强灵敏度和光谱灵活性的信号网络提供了强大的工具包.
    • 这些工具克服了以前的局限性,使细胞和组织中信号事件的定量映射成为可能.
    • 开发的传感器工具包阐明了信号网络的时空调节.