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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: May 7, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

蛋白质定量和物理规范化在蛋白质组学中总是必要的吗?

Alex Zelter, Michael Riffle, Gennifer E Merrihew

bioRxiv : the preprint server for biology

|February 23, 2026

概括

蛋白质定量通常被认为是液体染色学-质谱学/质谱学 (LC-MS/MS) 蛋白质组学必不可少的. 这项研究表明,省略物理规范化可以节省时间和成本,而不会显著影响计算规范化后的结果.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Published on: April 9, 2019

相关实验视频

Last Updated: May 7, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Published on: April 9, 2019

科学领域:

生物化学生物化学
分析化学分析化学
蛋白质组学是指蛋白质组学.

背景情况:

传统的蛋白质组学工作流程要求蛋白质量化和物理正常化.
这些步骤增加了大量的时间,成本和复杂性,特别是在大规模研究中.
计算规范化策略是蛋白质组学数据分析中的标准.

研究的目的:

调查遗漏物理蛋白质正常化对定量蛋白质组学数据的影响.
要确定计算规范化是否可以弥补缺少物理规范化的情况.
识别蛋白质组学工作流程中潜在的时间和成本节约.

主要方法:

来自样本的定量蛋白质组学数据的比较,有和没有物理蛋白质量正常化.
对和蛋白质丰度变化的分析.
标准计算规范化技术应用于规范化和非规范化数据集.

主要成果:

输入蛋白质量的变化增加与输出和蛋白质丰度的变化更大相关.
当运用计算规范化时,省略物理规范化并没有导致测量变量的不可接受的增加.
该研究确定了量化蛋白质组学中输入变异和输出变异之间的关系.

结论:

在某些定量蛋白质组学实验中,蛋白质的量化和物理规范化可能会被遗漏.
在许多情况下,计算规范化有效地弥补了缺乏物理规范化的情况.
这一发现使得蛋白质组学工作流程的时间和成本可以得到显著的优化.