相关实验视频
Updated: Feb 24, 2026

08:01
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
8.4K
贾格德1在内皮细胞机械转导中的非正规作用
Freddy Suarez Rodriguez1,2,3, Noora Virtanen1,2,3, Elmeri Kiviluoto1,2,3
1Faculty of Science and Engineering, Cell Biology, Åbo Akademi University, Turku, Finland.
The FEBS journal
|February 23, 2026
概括
机械力量通过Notch通路调节内皮细胞. 我们发现Jagged1,一个Notch连接体,控制机械敏感激酶如VEGFR2和ERK,独立于正规的Notch信号.
科学领域:
- 内皮细胞生物学 内皮细胞生物学
- 机械生物学 机械生物学
- 心血管生理学心血管生理学
背景情况:
- 诺奇信号通路对内皮细胞至关重要,并对血液动力学力作出反应.
- 机械力和Notch信号之间的集成机制尚未完全理解.
- Jagged1 是一个关键的Notch连接体,参与内皮细胞功能.
研究的目的:
- 研究Jagged1在内皮机械传导中的非正规作用.
- 阐明机械力是如何调节Jagged1及其下游信号的.
- 为了确定Jagged1是否影响机械敏感激酶,独立于正规的Notch激活.
主要方法:
- 研究了剪切应力对Jagged1表达和内皮细胞局部化的影响.
- 评估了Jagged1删除对VEGFR2和ERK活性在体外和体内 (斑马鱼) 的影响.
- 利用抗体结合的珠子来物理刺激Jagged1并观察下游的激酶激活.
主要成果:
- 剪切应力以大小依赖的方式诱导Jagged1的表达和转移到细胞结点.
- 删除Jagged1减少了VEGFR2和ERK活性,而刺激Jagged1则激活了这些激酶.
- Jagged1介导的激酶激活涉及原瘤基因氨酸蛋白激酶Src,独立于正规的Notch信号.
结论:
- Jagged1在调节内皮细胞机械转导方面发挥了新的非正规作用.
- Jagged1作为一个机械传感器,控制机械敏感激酶VEGFR2和ERK.
- 这一途径突出了机械力如何影响内皮细胞行为的新机制.
相关概念视频
Tension Response at Adherens Junctions
3.7K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
3.7K
Mechanism of Angiogenesis
7.2K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
7.2K
Cell-matrix's Response to Mechanical Forces
3.7K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
3.7K
Notch Signaling Pathway
6.7K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.7K
Regulation of Angiogenesis and Blood Supply
3.8K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.8K
Intracellular Signaling Affects Focal Adhesions
3.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
3.7K

