介质素-4改变了转录组,ECM相关成分和马子宫内膜纤维细胞的功能:从健康和纤维细胞的洞察力
Anna Wójtowicz1, Agnieszka Sadowska1, Kamil Myszczyński2,3
1Team of Molecular Basis of Equine Reproduction, InLife Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
Cell and tissue research
|February 23, 2026
概括
介质素-4 (IL-4) 在马子宫内膜纤维化中显著重编程纤维细胞. 这种免疫媒介影响基因表达,细胞行为和细胞外矩阵重塑,反应因纤维化阶段而异.
科学领域:
- 纤维化研究纤维化.
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 纤维化病原体尚未完全理解,尤其是免疫媒介在肌肉细胞编程中的作用.
- 马子宫内膜纤维化 (内膜纤维化) 提供了一个自发的大型动物模型来研究纤维化.
- 洲际蛋白-4 (IL-4) 是一种关键的免疫调解剂,在纤维化中具有潜在的作用.
研究的目的:
- 调查IL-4如何从健康和纤维化马体内膜重新编程纤维细胞.
- 确定IL-4对纤维细胞基因表达,细胞增殖,迁移和细胞外基质 (ECM) 重塑的影响.
- 探索纤维化阶段对IL-4的依赖性反应.
主要方法:
- 来自健康和受子宫内膜异位症影响的马子宫内膜的初级纤维细胞被培养并用IL-4进行治疗.
- 大量转录组学分析了48小时和96小时的基因表达变化.
- 进行了差异基因表达 (DEG) 和长非编码RNA (lncRNA) 分析.
- 进行了细胞增殖,活力和迁移试验.
- 评估了矩阵金属蛋白酶 (MMP) 的表达,丰度和活性.
主要成果:
- 在健康和纤维化子宫内膜的纤维细胞中,IL-4诱导显著差异性基因表达 (DEGs).
- 丰富分析将IL-4与改变的细胞代谢,ECM组织和纤维化途径联系起来.
- IL-4调节了lncRNA的表达,将它们与炎症,ECM组织和细胞因子信号连接起来.
- IL-4增加了纤维细胞的增殖和活力,但减少了健康的子宫内膜衍生细胞的迁移.
- IL-4以纤维化阶段依赖的方式改变了MMP表达和活性,影响了ECM周转.
结论:
- 在马体内膜纤维化中,IL-4作为纤维细胞功能的强大调节剂.
- 纤维细胞对IL-4的反应取决于起源组织的纤维化状态.
- 这项研究突出了IL-4在纤维化中的作用,并确定了ECM重塑的阶段依赖机制.
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