相关实验视频

Updated: Feb 24, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

在ssico转换ssRNA体到ssDNA:结构稳定性和形状保存的分子动态评估

Sabrina Lorenti¹, Nathalia Oliveira Alqualo¹, Danilo Caixeta Moreira¹

¹Laboratory of Nanobiotechnology Prof. Dr. Luiz Ricardo Goulart Filho, Institute of Biotechnology, Federal University of Uberlândia, Umuarama Campus, Avenida Pará, 1720, 38.400-902, Uberlândia, Minas Gerais, Brazil.

Journal of computer-aided molecular design

|February 23, 2026

概括

这项研究开发了一种计算方法,将不稳定的单链RNA (ssRNA) 受体转化为更稳定的单链DNA (ssDNA) 受体. 这些DNA版本保持了关键的结构特征,显示了治疗应用的前景.

科学领域:

生物化学生物化学
计算生物学计算生物学
分子生物物理学分子生物物理学

背景情况:

单链RNA (ssRNA) 受体具有高特异性,但遭受化学不稳定性和酶降解.
这种不稳定性限制了它们的临床应用,需要开发更强大的类似物.

研究的目的:

介绍一个计算工作流程,用于将ssRNA体转化为结构保存的单链DNA (ssDNA) 类似物.
在不同的热条件下评估这些ssDNA类型的结构稳定性.
为未来实验验证ssRNA转换为ssDNA的aptamer转换提供基础.

主要方法:

整合3D结构建模,有针对性的化学修改和广泛的分子动力学 (MD) 模拟 (200 ns在300 K和310 K).
利用MD描述符,如根-平均-平方偏差 (RMSD) 和分子内键进行结构比较.
专注于先前选择的前列腺癌细胞的吸收体 (A6和A11).

主要成果:

这些ssDNA类似物 (LN-A6和LN-A11) 成功保留了它们的ssRNA前体的关键结构特征.
在两个测试温度 (300 K和310 K) 上,ssDNA变异的形态行为保持一致.
虽然ssRNA体表现出更受限制的构造,但ssDNA类似物表现出足够的结构完整性.

结论:

开发的计算工作流可靠地评估在ssRNA转换为ssDNA聚酶过程中的结构保存.
ssDNA 体类似物是ssRNA 体的可行替代品,解决了稳定性问题.
这项研究为实验性努力奠定了基础,以创建更稳定的aptamer疗法.

关键词:

计算生物技术计算生物技术在化转换过程中.模拟分子动力学模拟前列腺癌是什么意思前列腺癌是什么意思结构稳定性结构稳定性这种 ssRNA 和 ssDNA 吸收剂.

更多相关视频

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

相关概念视频

Single-Strand DNA Binding Proteins

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...