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相关概念视频

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
775.8K
Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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相关实验视频

Updated: Feb 25, 2026

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

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下一代蛋白质测序和分析方法

Kingsley L-J Wong1,2, Mattias Tolhurst1,2, Oren A Fox1

  • 11Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, Washington, USA;

Annual review of analytical chemistry (Palo Alto, Calif.)
|February 23, 2026
PubMed
概括

新的蛋白质测序技术承诺通过链接序列,结构和功能来彻底改变蛋白质组学. 这些创新加速了生物发现和生物医学创新,克服了当前蛋白质分析方面的挑战.

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Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Sequencing of mRNA from Whole Blood using Nanopore Sequencing

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An Integrated Approach for Microprotein Identification and Sequence Analysis

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相关实验视频

Last Updated: Feb 25, 2026

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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

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科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 生物技术是生物技术.
  • 分子生物学分子生物学

背景情况:

  • 蛋白质组学面临的挑战是由于蛋白质的复杂性,放大困难和各种蛋白质形式.
  • 目前的大规模蛋白质组学研究主要依赖于质谱学.
  • 现有的方法很难在规模上全面地将蛋白质序列,结构和功能联系起来.

研究的目的:

  • 审查新兴的蛋白质测序和分析技术.
  • 评估新型蛋白质组技术的机制,进展和挑战.
  • 突出融合到下一代蛋白质基因平台的创新.

主要方法:

  • 测序,单分子测序,数字蛋白质组学映射和基于纳米孔的蛋白质测序的审查.
  • 对技术机制和当前发展阶段的分析.
  • 商业准备和未来潜力的评估.

主要成果:

  • 几种新技术提供了 de novo,单分子和更高通量蛋白质测序.
  • 测序,单分子测序,数字蛋白质组学映射和纳米孔测序正在接近商业化实施.
  • 这些进展有望克服当前蛋白质组方法的局限性.

结论:

  • 蛋白质测序的进步将改变蛋白质组学.
  • 这些技术将加速生物发现和生物医学创新.
  • 创新的融合预示着蛋白质组分析的新时代.