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对超衰减的S19脊髓灰质炎病毒菌株进行评估,用于脊髓灰质炎病毒中和试验
Nicholas Wiese1,2, William Hendley1, Basit Jafri1
1Polio and Picornavirus Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GeorgiaUnited States of America.
PloS one
|February 23, 2026
概括
新的超减弱型脊髓灰质炎病毒菌株 (S19-PV) 在抗原上与参考病毒相当. 这些菌株允许在高制实验室之外更广泛地获得脊髓灰质炎病毒血清检测,有助于根除工作.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 公共卫生 公共卫生
背景情况:
- 脊髓灰质炎病毒的根除依赖于疫苗接种和实验室制.
- 血清检测对于监测人口免疫力和疫苗疗效至关重要.
- 目前针对特定的脊髓灰质炎病毒菌株的封闭要求限制了血清学测试的可访问性.
研究的目的:
- 评估超衰减的,对温度敏感的S19脊髓灰质炎病毒菌株 (S19-PV) 进行血清学检测.
- 评估S19-PVs与萨宾和野生型脊髓灰质炎病毒菌株的抗原等价性.
- 确定S19-PVs在生物安全级别2 (BSL-2) 条件下适用于中和试验的适用性.
主要方法:
- 对S19-PVs与Sabin和野生型脊髓灰质炎基准菌株进行比较验证.
- 使用了经过修改的微中和试验.
- 采用世卫组织的国际标准,内部控制和人类血清进行验证.
主要成果:
- 在中和抗体标位上观察到S19-PV和参考菌株之间的强烈一致性 (R2 = 0.93-0.99).
- 证明了高的测试内和测试间可重复性 (CVs<10%).
- 证实S19-PV菌株在抗原上相当于萨宾和野生型病毒.
结论:
- S19-PV菌株适用于严格制之外的脊髓灰质炎病毒中和抗体测定.
- 采用S19-PVs可以扩大全球脊髓灰质炎病毒血清检测能力.
- 这有助于在脊髓灰质炎病毒根除中开展监测和免疫活动.
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