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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Updated: May 8, 2026

An Improved and High Throughput Respiratory Syncytial Virus RSV Micro-neutralization Assay

An Improved and High Throughput Respiratory Syncytial Virus RSV Micro-neutralization Assay

Published on: January 26, 2019

一个LC-免疫-MRM-MS冬季复合体方法开发框架用于呼吸道病毒查.

Bart Van Puyvelde¹, François E Dufrasne², Sarah Denayer²

¹ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, 9000 Ghent, Belgium.

Analytical chemistry

|February 23, 2026

概括

一种新的测定方法,Winterplex,可以同时检测流感A,流感B,SARS-CoV-2和RSV. 这种基于质谱的方法为RT-qPCR提供了一个可扩展的替代方案,用于呼吸道病毒监测和流行病准备.

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Remote Laboratory Management: Respiratory Virus Diagnostics

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An Improved and High Throughput Respiratory Syncytial Virus RSV Micro-neutralization Assay

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Published on: January 26, 2019

Remote Laboratory Management: Respiratory Virus Diagnostics

Remote Laboratory Management: Respiratory Virus Diagnostics

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Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

Published on: November 10, 2023

科学领域:

临床诊断临床诊断临床诊断
病毒学病毒学
质谱测量质量谱测量

背景情况:

对流感,SARS-CoV-2和RSV等呼吸道病毒进行可靠的大规模测试对于监测和流行病应对至关重要.
RT-qPCR是黄金标准,但COVID-19大流行揭示了需要可扩展的替代检测平台的需求.
基于质谱法 (MS) 的方法对多重病毒检测有希望,但需要临床验证.

研究的目的:

开发和验证一种用于同时检测多个呼吸道病毒的新型检测方法.
评估新试验的性能与当前的黄金标准 (RT-qPCR) 相比.
突出基于多发性硬化症的诊断对于未来的流行病准备的潜力.

主要方法:

开发一种名为Winterplex的定量免疫多重反应监测 (iMRM) 试验.
同时检测A型流感,B型流感,RSV和SARS-CoV-2,每种病毒使用两个蛋白型.
根据ISO标准对体外诊断和对RT-qPCR进行基准测试的验证.

主要成果:

温特普莱克斯试验证明了成功同时检测目标呼吸道病毒.
该试验的性能与RT-qPCR进行了比较,表明其作为替代检测方法的可行性.
根据ISO标准的验证证实其适用于体外诊断.

结论:

温特普莱克斯试验为多重呼吸道病毒检测提供了一个经过验证的,可扩展的平台.
投资翻译和扩展基于多重多发性多发性硬化症的诊断方法对于加强流行病准备是必不可少的.
基于多发性硬化症的诊断提供了结合多种目标的灵活性,使得对新出现的病毒威胁作出快速反应.