布拉赫玛抑制了STOP1-NRT1.1模块,以控制植物根系球的化和酸性应激适应
Jia Yuan Ye1, Wen Hao Tian2, De Rui Zhang1
1State Key Laboratory of Plant Environmental Resilience, College of Life Science, Zhejiang University, Hangzhou, 310058, China.
Nature communications
|February 23, 2026
概括
布拉玛 (BRM) 蛋白通过抑制 STOP1-NRT1.1 途径来抑制阿拉比多普西斯中的酸性应激耐受性. 低pH触发BRM降解,激活这种途径以改善吸收和酸性土壤中的根生长.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 土壤科学 土壤科学
背景情况:
- 酸性土壤覆盖着广的耕地,通过阻碍根部发育和营养吸收来限制作物生产率.
- 阿拉比多普西斯的转录因子STOP1对于减轻酸性压力至关重要,通过NRT1.1.1.促进酸盐 (NO3-) 吸收和根球化.
- 在酸性条件下STOP1-NRT1.1路径的上游调节还不清楚.
研究的目的:
- 为了确定Arabidopsis中对pH响应的STOP1-NRT1.1通路的关键调节者.
- 阐明BRAHMA (BRM) 影响酸性应激耐受性的机制.
- 了解在酸性土壤条件下NO3的获取是如何与pH平衡结合在一起的.
主要方法:
- 酵母二杂交和共免疫沉试验验证实了物理相互作用.
- 染色体免疫沉降测序 (ChIP-seq) 用于识别BRM结合部位.
- 使用单个和双重突变 (brm,stop1,nrt1.1) 的基因分析来确定表现关系.
- 定量实时PCR (qRT-PCR) 用于评估基因表达水平.
主要成果:
- BRAHMA (BRM) 是一种SWI2/SNF2类型的ATPase,被确定为STOP1-NRT1.1通路的抑制剂.
- BRM与STOP1进行物理相互作用,并与NRT1.1促进体结合,抑制STOP1介导的NRT1.1表达和随后的NO3吸收.
- 低pH迅速诱导BRM降解,缓解抑制,并使STOP1-NRT1.1通路的强大激活,从而提高根生长和使用效率 (NUE).
结论:
- BRM-STOP1-NRT1.1轴代表了一个集成酸盐获取和pH平衡的中央调节模块.
- 在低pH下BRM的动态降解对于激活酸性应激耐受机制至关重要.
- 这一监管轴为改善酸性土壤中的作物弹性和通过增强NUE减少肥料诱导的土壤酸化提供了潜在的战略.
相关概念视频
Cell Signaling in Plants
6.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.7K
Repressible Operon: trp Operon
2.0K
The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
2.0K
Adaptations that Reduce Water Loss
28.3K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
28.3K
Prokaryotic Transcriptional Activators and Repressors
25.7K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
25.7K
Prokaryotic Transcriptional Activators and Repressors
10.8K
10.8K
Regulation of Transpiration by Stomata
31.5K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
31.5K


