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激活ABF2的sly-miR398 中介于番茄中ABA-依赖的超氧化物积累和胃关闭

Xijiao Zhai¹, Lei Ru^2,3, Guochao Yan^2,3

¹College of Horticulture, Shanxi Agricultural University, Taigu 030801, Shanxi, China.

Journal of experimental botany

|February 24, 2026

概括

酸 (ABA) 通过活性氧物种 (ROS) 触发植物干旱反应. 这项研究揭示了SnRK2.6-ABF2-miR398b-CSD1通路微调ROS水平,控制口腔关闭并增强番茄的干旱耐受性.

科学领域:

植物生物学植物生物学
分子植物生理学分子植物生理学
生物化学生物化学

背景情况:

酸 (ABA) 对于植物干旱反应至关重要,涉及反应性氧物种 (ROS) 信号传递.
抗氧化酶及其调节剂在ABA介导的口腔关闭中的精确作用尚未完全理解.

研究的目的:

研究miR398及其点SlCSD1在番茄 (Solanum lycopersicum) 中ABA诱导的口腔关闭和耐干旱的功能.
阐明涉及ABA,ROS和口腔孔径控制的调节途径.

主要方法:

基因表达分析 (sly-miR398b和SlCSD1的过度表达和淘汰突变).
测量超氧化物离子 (O2•−) 水平.
药理学测试以评估ROS依赖性.
对转录因子结合 (SlABF2) 和促进因子活性的分析.
通过SlSnRK2.6.6对上游监管进行调查

主要成果:

过度表达sly-miR398b或SlCSD1淘汰会增加O2•−,促进口腔关闭,并增强干旱耐受性.
缺乏sly-miR398b的突变体呈现出减少的O2•−,受损的口腔关闭,以及增加干旱敏感度.
SlABF2直接激活了sly-miR398b的表达,而SlSnRK2.6调节了SLABF2的活动.
确定了SnRK2.6-ABF2-miR398b-CSD1模块是O2•−水平和口腔孔径的关键调节器.

结论:

SnRK2.6-ABF2-miR398b-CSD1调控级联微调ROS水平以控制口腔关闭.
这种途径在提高番茄植物对干旱的耐受性方面发挥着重要作用.

关键词:

亚酸亚酸是什么干旱干旱干旱干旱反应性氧物种的反应性氧物种.胃口关闭胃口关闭这就是为什么 miR3988.

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