昆明小鼠的微观规模肠道微生物群数据集通过16S rRNA基因高通量测序揭示了16S rRNA基因的高通量测序
Ruibiao Fu1,2, Jin Li2, Meng Wang3
1Department of General Surgery, The First Affiliated Hospital of Soochow University, 215006, Suzhou, China.
Data in brief
|February 24, 2026
概括
这项研究揭示了微量和散装肠道样本之间的肠道微生物组成和复杂性的显著差异. 分析结肠粒可提供微生物空间组织的高分辨率视图.
科学领域:
- 微生物组研究的研究.
- 肠道微生物生态学
- 空间转录组学 空间转录组学
背景情况:
- 肠道微生物群显著影响宿主生理.
- 之前的研究往往忽略了微小规模的微生物异质性,因为它们依赖大量样本.
研究的目的:
- 在100微米以下的尺度上研究肠道微生物空间组织.
- 为了比较微生物群落结构之间的微规模的结肠粒和批量样本.
- 为微生物组研究提供高分辨率数据集.
主要方法:
- 从昆明小鼠收集了378个结肠微量粒 (20-40微米) 和20个批量样本.
- 使用Illumina NovaSeq 6000进行了16S rRNA V4区域测序.
- 处理了超过800万个读取到1506个安普利康序列变体 (ASV).
主要成果:
- 确定了19个类和203个属,其中Firmicutes和Bacteroidetes占主导地位.
- 在微量和大批量样本之间观察到ASV丰度 (p < 0.001) 和网络复杂性 (p = 0.03) 的显著差异.
- 产生了肠道微生物空间组织的高分辨率数据集.
结论:
- 微尺度分析揭示了与批量样本相比,不同的肠道微生物社区结构.
- 该数据集促进了对微生物组合,物种间相互作用和规模依赖的社区结构的理解.
- 这个资源支持未来的微生物生态学和机理学研究.
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