克拉米迪亚形虫培养,活力测试和测序方法的叙述性审查
Alana K Sterkel1, Travis J Loya2, Amanda C Smith2
1Wisconsin State Laboratory of Hygiene, University of Wisconsin-Madison, Madison, Wisconsin, USA.
概括
克拉米迪亚甲状腺虫 (CT) 培养的进展有限,阻碍了抗菌感应性测试 (AST) 和生存能力评估. 未来的研究需要标准化的诊断和抗药性监测方法.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 甲状腺炎 (CT) 是美国普遍存在的细菌性性传播疾病,通常由NAAT检测.
- CT的细胞内性质使培养,生命力确定和抗菌素敏感性测试 (AST) 复杂化.
- 文化对于菌株类型,治愈测试,性侵犯案件和抗药性监测至关重要.
研究的目的:
- 系统地审查CT培养方法的进展.
- 评估CT的生存能力确定和抗菌素耐药性检测方面的进展.
- 确定当前技术的差距,并指导未来的研究.
主要方法:
- 在5个电子数据库中进行了系统的文献搜索.
- 关键词集中在CT培养,生命力测定和抗菌素耐药性检测方法上.
- 鉴定了29个相关的手稿,并进行了叙事合成.
主要成果:
- 在过去十年中,CT培养技术的进展有限.
- 有希望的可行性和耐药性检测方法缺乏标准化和广泛使用.
- 现型AST仅限于专业实验室;验证的基因型检测很少,阻碍了对抗性趋势的评估.
结论:
- 虽然CT培养的临床作用正在减少,但它仍然对研究和监测至关重要.
- 关键的技术差距包括区分可行的和不可行的CT和标准化的AST协议.
- 未来的研究应该专注于临床可行性测试,共识AST方法和综合基因组方法.
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