相关实验视频
Updated: Feb 26, 2026

09:25
Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain
Published on: May 21, 2019
7.3K
在聚乙烯纳米塑料诱导的空间认知功能障碍中,通过微质激活诱导的神经元亡的全方位化的一个新机制
Qing Du1, Ning Bu1, Xuan Zhou2
1Center for Global Health, The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Suzhou Institute for Advanced Study of Public Health, Gusu School, Nanjing Medical University, Nanjing 211166, Jiangsu, PR China.
Journal of hazardous materials
|February 24, 2026
概括
聚乙烯纳米颗粒 (PS-NPs) 通过破坏微质脂代谢并引起神经炎症,导致认知缺陷,从而损害大脑. 这项研究确定了治疗纳米塑料诱导的神经损伤的潜在目标.
科学领域:
- 神经科学是一个神经科学.
- 毒理学 毒理学 毒理学
- 生物化学 生物化学
背景情况:
- 越来越多的证据将纳米塑料 (NP),特别是聚乙烯纳米粒子 (PS-NP) 与神经毒性联系起来.
- 已知PS-NPs可以穿过血脑屏障,并在海马中积累.
- 观察到的神经毒性机制包括氧化应激,神经炎症和突触损伤.
研究的目的:
- 研究通过PS-NPs在海马中诱导神经毒性的分子机制.
- 阐明微质脂质代谢在PS-NP诱导的神经炎症和神经元损伤中的作用.
- 确定纳米塑料诱导的神经疾病的潜在治疗点.
主要方法:
- 在体外和体外模型被用来研究PS-NP对微质和神经元的影响.
- 分析了关键的分子通路,包括RNF139,SCAP,SREBP和脂质代谢.
- 评估了线粒体功能,炎症性细胞因子释放和神经元亡.
- 在暴露于PS-NPs的动物模型中评估了空间认知功能.
主要成果:
- 发现PS-NP可以降低微质中的RNF139的调节,从而损害SCAP降解.
- 升高的SCAP水平导致SREBP激活,改变了脂质代谢,并增加了脂质合成.
- 观察到线粒体功能障碍,反应性氧物种增加和ATP合成减少.
- 激活的微质分泌出促炎性细胞因子 (TNF-α,IL-1β,IL-6),诱导神经元损伤和亡.
- 暴露于PS-NP导致空间认知障碍.
结论:
- 暴露于PS-NP会触发一连串的过程,包括微质脂质失调,神经炎症和神经元损伤.
- RNF139和SCAP-SREBP通路是PS-NP神经毒性的关键调解者.
- 微质脂质代谢的破坏是纳米塑料诱导的神经退行和认知障碍的关键因素.
- 针对这些途径可能为治疗纳米塑料引起的神经系统疾病提供一种策略.
更多相关视频
相关概念视频
The Proteasome
1.8K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
1.8K
The Proteasome
10.4K
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
10.4K
Regulated Protein Degradation
9.0K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
9.0K
Destabilization of Microtubules
3.8K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
3.8K
Receptor Downregulation in MVBs
2.9K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.9K

