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最近使用的具有改性单体组合的通用粘合剂对人类牙上皮细胞的潜在细胞毒性作用

Omar Abd El-Maksoud¹, Nessma Sultan^2,3, Hoda Saleh Ismail⁴

¹Conservative Dentistry Department, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Gamasa, Egypt.

Scientific reports

|February 24, 2026

概括

三种新的通用粘合剂 (UA) 在人类牙细胞上表现出不同的细胞毒性. 修改后的配方可能会提供更安全的配置文件,Clearfil TRI-S Bond Universal Quick (CSQ) 显示出最好的细胞兼容性.

科学领域:

牙科材料科学牙科材料科学
生物材料是一种生物材料.
细胞生物学细胞生物学

背景情况:

全面粘合剂 (UA) 在牙科中广泛使用.
有关单体的细胞毒性存在担忧,比如双甲-甘油甲基酸盐 (Bis-GMA) 和乙烯甲基酸盐 (HEMA).
新的UA中修改的单体化合物可能会影响它们的生物安全性.

研究的目的:

为了评估和比较三种近期的普遍粘合剂 (UA) 与修饰的单体化合物的体内细胞毒性.
评估这些UA对人类牙上皮细胞的影响.
将它们的细胞毒性与含有Bis-GMA和HEMA的对照粘合剂进行比较.

主要方法:

准备了Scotchbond Universal Plus (SBP),Zipbond Universal (ZBU),Clearfil TRI-S Bond Universal Quick (CSQ) 和Tetric N-Bond Universal (TNB) 的样本,这些样本的使用情况如下:
提取介质是通过在Dulbecco的修饰介质 (DMEM) 中化标本而获得的.
用MTT,SRB和活性氧物种 (ROS) 试验在50%和100%度下评估细胞毒性.

主要成果:

与对照组相比,MTT测定表明SBP,ZBU和TNB的细胞活力显著降低,而CSQ显示活力最高.
SRB测试显示,SBP,ZBU和CSQ的细胞活力与对照组相似,明显高于TNB.
与对照组和彼此相比,所有测试的UA中ROS含量存在显著差异.

结论:

通用粘合剂的细胞毒性取决于它们的化学成分和度.
在UA中修改的单体配方,如CSQ中,似乎增强了细胞兼容性.
对单体修饰的进一步研究是有必要的,以开发更安全的牙粘合剂.

关键词:

生物相容性生物相容性细胞活力细胞活力细胞毒性细胞毒性牙上皮质细胞牙上皮质细胞全面的粘合剂是普遍的粘合剂.

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