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Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Updated: Feb 26, 2026

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
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Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

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卷度DNA显微镜用于在三维中绘制空间转录组的映射.

Nianchao Qian1, Jing Li1, Reem Yasser2

  • 1Department of Medicine, Section of Genetic Medicine, University of Chicago, Chicago, IL, USA.

Nature protocols
|February 24, 2026
PubMed
概括
此摘要是机器生成的。

卷度DNA显微镜分析无光学的完整组织中的空间转录组. 这种可扩展的方法使用DNA编码和测序来重建3D细胞组织和微环境.

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

背景情况:

  • 生物系统本质上是3D的,但目前的空间转录组学方法仅限于2D组织部分.
  • 这种限制阻碍了完整组织体内的细胞组织和微环境的分辨率.

研究的目的:

  • 开发一种可扩展的,无光学方法,直接在完好无损的生物标本中进行空间转录组分析.
  • 为了使三维空间关系的重建能够进行全面的组织分析.

主要方法:

  • 开发了体积DNA显微镜,将空间信息编码到分子间网络中的DNA分子中.
  • 详细的工作流程:在现场cDNA合成,用于空间编码的DNA纳米球形成,近距离桥接和通过地质谱嵌入进行3D重建.
  • 使用常规的分子生物学试剂和台式测序器用于库生成和计算分析.

主要成果:

  • 在完整的组织中成功建立了体积空间转录组概况的完整工作流.
  • 通过DNA编码和短读测序来重建三维空间关系的能力.
  • 工作流是高效的,序列库在7-8天内生成.

结论:

  • 卷度DNA显微镜为探索完整组织中的遗传和形态特征提供了一个多功能平台.
  • 克服了2D组织切割的局限性,为组织结构和细胞相互作用提供了更深入的见解.
  • 为使用标准分子生物学技术和设备的研究人员提供可访问的技术.