Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Histone Modification02:32

Histone Modification

16.6K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
16.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling.

Science advances·2026
Same author

The Development of New SSR Markers and an Assay for Genotyping Sweet Cherry (<i>Prunus avium</i> L.) in One Reaction.

International journal of molecular sciences·2026
Same author

Introgression of barley chromosome arms 4H and 6H into wheat via Robertsonian translocations: GBS-assisted structural analysis and impact on grain nutrient composition.

Plant molecular biology·2026
Same author

Sorghum embryos undergoing B chromosome elimination express B-variants of mitotic-related genes.

Genome biology·2025
Same author

Combined action of suicide gene exosomes from pancreatic cancer-associated fibroblasts and from mesenchymal stem cells as a pancreatic ductal adenocarcinoma treatment approach.

Cancer cell international·2025
Same author

Thermal Plasticity of Multiple Traits Varies More Within Than Between Populations of <i>Plantago lanceolata</i> at Its Northern Range Edge.

Ecology and evolution·2025

相关实验视频

Updated: Feb 26, 2026

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
07:10

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Published on: March 4, 2021

5.1K

增强的植物自下而上的希斯蛋白质组学

Palina Ryzhaya1,2, Pavlína Pírek1, Radomír Pech1

  • 1Mendel Center for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic.

Journal of experimental botany
|February 24, 2026
PubMed
概括

这项研究通过优化样本准备,通过使用质谱 (MS) 增强了植物质子分析. 新的工作流程产生纯素提取物,用于在作物中进行详细的表观遗传研究.

关键词:
齐亚梅斯 (Zea mays) 是一种植物.农作物 植物 植物流动细胞计量是流动细胞计量的方法.基因组的衍生化 基因组衍生化基因组 基因组 基因组质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量翻译后的修改 翻译后的修改蛋白质组学 蛋白质组学三甲基酸盐无水化物

更多相关视频

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

30.6K
Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
09:48

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Published on: April 22, 2022

4.5K

相关实验视频

Last Updated: Feb 26, 2026

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
07:10

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Published on: March 4, 2021

5.1K
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

30.6K
Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
09:48

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Published on: April 22, 2022

4.5K

科学领域:

  • 植物生物学 植物生物学
  • 蛋白质组学是指蛋白质组学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 对植物表观遗传学和生物技术的理解来说,质蛋白形状的表征是至关重要的.
  • "自下而上的蛋白质组学"提供了一种方法来研究植物染色素的表观遗传特征.
  • 质谱学 (MS) 分析质需要仔细的样本准备,包括化学衍生,以实现最佳的纳米HPLC分离.

研究的目的:

  • 为了评估来自Zea mays叶的不同组素制备协议,以提高质谱 (MS) 数据质量.
  • 增强现有的基于MS的植物质子分析协议,以更好地表征质子蛋白形态.

主要方法:

  • 从Zea mays树叶中优化组织蛋白提取.
  • 使用三甲基酸盐无水化物对氨基群的化学衍生.
  • 使用一种新型蛋白酶Arg-C Ultra的酶性消化.
  • 光辅助细胞分类 (FACS) 用于纯色素样本的分离.

主要成果:

  • 开发了一种结合化学衍生和酶消化的增强协议,用于植物质子分析.
  • 证明了FACS在获取纯色素样本方面的有效性,绕过了传统的沉方法.
  • 获得了高度纯净的素提取物,适用于对翻译后修饰和变异组合的定量分析.

结论:

  • 拟议的工作流程显著提高了用于植物质子分析的质谱数据的质量.
  • 这些方法为研究农业重要作物的表观遗传模式和动态提供了强大的工具.
  • 增强的质子表征促进了植物生物技术和作物改良方面的进步.