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由 OsSRWD1,OsRACK1A 和 OsRGLG2 控制的 ONAC122 和 ONAC131 通过调节 OsRFPH2-2 和 OsBIERF3 来调节大米免疫力

Yan Bi^1,2,3, Leeza Tariq^1,2,3, Yuqing Yan^1,2,3,4

¹National Key Laboratory for Rice Biology and Breeding, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China.

The New phytologist

|February 25, 2026

概括

米的免疫包括NAM,ATAF1/2和CUC2 (NAC) 转录因子. 通过激活免疫基因,ONAC122和ONAC131通过激活其他调节性蛋白质调节其活性来积极调节水爆耐药性.

科学领域:

植物分子生物学植物分子生物学
植物病理学植物病理学
遗传学是一个遗传学.

背景情况:

NAM,ATAF1/2和CUC2 (NAC) 转录因子对于植物免疫是至关重要的.
众所周知,ONAC122和ONAC131可以增强米粉的抗爆性能,但它们的调节机制尚不清楚.

研究的目的:

阐明ONAC122和ONAC131调节大米免疫力的分子机制.
为了确定目标基因和ONAC122和ONAC131.1.的交互伙伴.

主要方法:

使用过度表达和淘汰赛大米线的基因表达分析.
电泳运动转移试验 (EMSAs) 用于确定DNA结合特异性.
共同免疫沉和酵母双杂交试验以确定相互作用的蛋白质.

主要成果:

ONAC122和ONAC131积极调节大米免疫力,并向免疫相关基因.
ONAC122与CACG和ACTC的cis元素结合,而ONAC131与CACG的cis元素结合.
ONAC122激活了OsRFPH2-2和OsBIERF3的转录,而ONAC131则协同增强了这种激活.
OsRGLG2在降解过程中使ONAC122和ONAC131无处不在,作为负调节剂.

结论:

一个涉及ONAC122,ONAC131及其相互作用伙伴 (OsRGLG2,OsSRWD1,OsRACK1A) 的调控模块可以积极控制大米免疫力.
该模块激活了关键免疫调节剂OsRFPH2-2和OsBIERF3的转录,增强了米爆耐药性.

关键词:

马格纳波特花 (Magnaporthe oryzae) 是一个很大的植物.在 ONAC12222 中.在ONAC131中,我们可以看到ONAC131.OsRFPH2‐2 的情况.这是OsRGLG2的.这种元素是cis元素.大米免疫力免疫力

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