高压均质化修改了Pediococcus acidilactici S1外聚糖的结构,并提高了其胆盐结合能力
Xiaohui Yang1,2,3, Di Bao3, Yifan Zhang1,2,3
1Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization of Ministry of Agriculture and Rural Affairs, Hefei, China.
Journal of food science
|February 25, 2026
概括
高压均质化增强了Pediococcus acidilactici S1外聚糖 (EPS),以提高胆盐的结合能力,降低胆固醇. 这种修改后的EPS显示出作为一种功能成分的潜力,可以自然降低胆固醇.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 胆盐 (BSs) 的结合能力对于降低胆固醇至关重要.
- 来自Pediococcus acidilactici S1 (S1-D1N1) 的外聚糖 (EPS) 具有潜在的降胆固醇特性.
- 物理修改方法可以增强EPS的功能.
研究的目的:
- 使用高压均质化 (HPH) 增强Pediococcus acidilactici S1外聚糖 (S1-D1N1) 的胆盐结合能力.
- 为了研究由HPH修饰引起的结构和组成变化.
- 评估HPH修饰的S1-D1N1作为胆固醇管理的功能成分的潜力.
主要方法:
- 使用响应表面方法 (RSM) 来优化HPH参数 (度,压力,循环).
- 修改后的外聚糖 (H-S1-D1N1) 分析了胆盐的结合能力.
- 结构和组成分析包括HPAEC,SEM,FT-IR,甲基化分析和分子对接.
主要成果:
- 最佳的HPH参数 (60 mg/mL S1-D1N1,40 MPa,3个周期) 显著提高了BSs的结合能力.
- HPH诱导的结构变化:曼诺含量增加,碳水化合物成分改变,结构更光滑/更薄,结合减少,暴露的疏水性群体.
- 分子对接证实了曼诺斯残留物作为胆酸盐的主要结合点.
结论:
- 修改HPH有效地提高了S1-D1N1.1.的胆盐结合能力.
- 增强的结合归因于曼诺含量增加,结构重组和功能组暴露.
- H-S1-D1N1是一种有希望的功能成分,可用于食品和补充剂中的降胆固醇应用.
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