与吸烟和COPD相关的致病性细胞外囊泡的自我传播性质
Camilla Margaroli1,2, Kristopher R Genschmer2,3, Ningyong Xu3
1Division of Molecular and Cellular Pathology, Department of Pathology, The University of Alabama at Birmingham, Birmingham, AL, United States.
来自慢性阻塞性肺病 (COPD) 患者的中性细胞弹性酶阳性细胞外囊 (NE+ EVs) 可以自我传播并导致慢性肺炎. 这种自我传播机制有助于COPD和吸烟者的肺气和慢性炎症.
科学领域:
- 肺部医学 肺部医学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 中性粒细胞 (PMN) 衍生的中性粒细胞弹性酶阳性 (NE+) 细胞外囊泡 (EVs) 导致COPD病理,包括肺气.
- 慢性PMN炎症是COPD的关键特征,但PMN招募的机制尚未完全理解.
研究的目的:
- 调查PMN衍生的NE+电动汽车是否可以产生额外的NE+电动汽车.
- 为了确定NE+EV是否促进慢性PMN炎症.
主要方法:
- 人类中性粒细胞被刺激在体外产生NE+和NE-EV.
- 电动汽车从暴露在香烟烟雾中的小鼠中分离出来.
- EVs被转移到幼稚的小鼠中,PMN衍生的EVs在7天后被分离出来.
- 为了评估自我传播,进行了连续EV转移.
主要成果:
- 激活的人类PMN衍生的NE + EVs诱导了小鼠PMN衍生的NE + EVs在小鼠呼吸道中的新生代.
- 这些小鼠NE + EVs通过多个通道连续传播肺气和NE + EV生产.
- 这种传播是由小鼠NE+EVs产生Proline-Glycine-Proline (PGP) 的媒介,它招募和激活PMN,导致更多的NE+EV释放.
- 烟雾引起的电动汽车也连续传播了肺瘤.
结论:
- 由PMN衍生出的NE+EVs在COPD和吸烟者中充当自我传播剂.
- 这些发现为COPD慢性炎症机制提供了新的见解.
- EVs可能在COPD和慢性炎症性肺部疾病的发病过程中发挥关键作用.
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