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使用并行光共聚焦显微镜检测前列腺腺癌的前列腺腺癌

Kevin Clare¹, Kenneth Haines¹, Jeremy Littlefield¹

¹Department of Pathology, Molecular and Cell-base Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

BJU international

|February 25, 2026

概括

这项研究确定了使用光共聚焦显微镜 (FCM) 诊断前列腺癌的标准. 它提供了一个图像图谱,以帮助病理学家在前列腺切除术期间快速进行手术内边缘评估.

科学领域:

尿路病理学尿路病理学
数字病理学数字病理学
显微镜的使用方法

背景情况:

准确诊断前列腺腺癌对于患者的管理至关重要.
在急性前列腺切除术期间,对手术边缘的术内评估是必不可少的.

研究的目的:

使用光共聚焦显微镜 (FCM) 创建前列腺腺癌及其变体的组织学目录和诊断标准.
为了能够熟练使用FCM进行手术边缘的快速手术内评估.

主要方法:

连续切割和FCM扫描22个急性前列腺切除标本.
将FCM图像与传统的血素和色素染色组织学进行比较.
对良性和恶性前列腺组织的FCM特征的评估.

主要成果:

为良性和恶性前列腺组织开发了一个全面的FCM图像目录.
癌症腺体的明确FCM特征被确定为:结合/连接的腺体,缺乏光度,异质的核信号,缺乏3D深度.
在FCM上观察到格里森模式3和4之间的形态差异.
最小的可检测癌症焦点是0.7 x 1.2毫米 (格里森得分6).

结论:

开发的图像图谱和诊断标准促进了病理学家在FCM方面的培训.
FCM可以有效地用于急性前列腺切除术中手术边缘的快速手术内评估.

关键词:

光共聚焦显微镜光共聚焦显微镜在手术内进行的评估.正常的组织学.前列腺腺腺癌前列腺腺腺癌.进行激进的前列腺切除术.

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