干血斑点卡的分析验证,用于评估COVID-19病毒阳性
Claire Auger1, Imad Tarhoni1, Grant Lewin1
1Department of Anatomy & Cell Biology, Rush University Medical Center, Chicago, IL, United States.
The journal of applied laboratory medicine
|February 25, 2026
概括
干血斑点 (DBS) 卡为COVID-19血清检测提供了一个稳定,具有成本效益的替代方案. 这种转换保持了高检测准确度的抗体检测长达四个月,简化样品处理.
科学领域:
- 免疫学 免疫学 免疫学
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 高灵敏度血清检测测定量与微量采样技术兼容.
- 一个COVID-19免疫体检测成功地从血适应到干血斑点 (DBS) 卡上.
研究的目的:
- 使用DBS矩阵评估COVID-19免疫体试验的分析性能和稳定性.
- 为了比较DBS卡的准确性和可靠性与传统的血样本进行血清学检测.
主要方法:
- 用COVID-19病毒阳性和病毒阴性患者的血样本应用于DBS卡.
- 分析物的稳定性在室温下经过120天的评估.
- Luminex测定测量了抗体标位 (Spike和Nucleocapsid糖蛋白);强大的线性模型预测了矩阵水平.
主要成果:
- DBS测定精度为94.9%,与血可比 (<1%的差异).
- 总IgG正常化提高了DBS准确度,达到97.3%.
- 在120天内观察到抗体标位的优异一致 (>0.9),证明了分析剂的稳定性.
结论:
- DBS卡提供了一种方便且具有成本效益的血清样本采集方法.
- 在DBS卡中,分析物的稳定性在不影响诊断性能的情况下被证实长达4个月.
相关概念视频
02:45Blood Transfusion and Agglutination
13.0K
Blood transfusion is a therapeutic measure to restore the blood volume after extensive blood loss due to an accident or a medical procedure. Blood transfusion involves drawing a certain amount of blood from a suitable donor and infusing it into the recipient.
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
13.0K
01:33Enzyme-Linked Immunosorbent Assay
14.1K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
14.1K
01:10Blood Typing
5.9K
Understanding an individual's blood group is a critical component of transfusion medicine. It ensures compatibility in blood transfusions, organ transplants, and even during pregnancy. Determining these blood groups involves the ABO and Rh blood typing systems, utilizing specific antigens and corresponding anti-sera to identify an individual's blood type.
Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target...
Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target...
5.9K