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通过16S rRNA识别和体外功能表征来探索婴儿肠道衍生的益生菌

Ishita Modasiya¹, Ritesh Nasit², Harsh Purohit¹

¹Postbiotics and Foodomics Lab, Department of Microbiology, School of Science, RK University, Rajkot, Gujarat, India.

|February 25, 2026

概括

两个有益的细菌菌株,Lb. 帕拉凯西 IF5YR 和 Ent. 从婴儿肠道微生物群中分离出来的体IF5RW2,显示出作为益生菌的前景. 它们表现出优异的胃肠性和抗氧化特性,用于肠道健康应用.

科学领域:

微生物学微生物学
肠道微生物组研究研究
益生菌开发益生菌开发

背景情况:

婴儿肠道微生物群在健康方面发挥着至关重要的作用,影响消化,免疫和新陈代谢.
从这个来源识别有益的微生物是开发新型益生菌的关键.
益生菌提供潜在的健康益处,包括改善肠道健康和免疫功能.

研究的目的:

从婴儿便样本中识别和描述潜在的益生菌细菌分离物.
为了评估所选单独的胃肠道弹性和功能性质.
确认有前途的益生菌候选者的安全性和分类属性.

主要方法:

从婴儿便样本中选47种细菌分离物.
16S rRNA测序用于细菌识别和遗传学分析.
在体外检测酸/胆耐受性,自身聚合,水性,胆盐酸酶活性和抗氧化能力 (FRAP,ABTS,DPPH).
用于安全评估的抗生素敏感性测试.

主要成果:

两个分离物,Lb. 帕拉凯西 IF5YR 和 Ent. 体IF5RW2被确定为有前途的候选物.
这两种菌株都表现出对酸 (pH1.5) 和胆汁 (2%) 的强烈耐受性.
观察到高自聚 (>70%) 和细胞表面水性 (>90%).
证实了显著的胆盐酸酶活性和强大的抗氧化能力 (FRAP>75%).
对抗生素敏感性测试表明,这两种隔离品的安全概况.

结论:

一个英. 帕拉凯西 IF5YR 和 Ent. 体IF5RW2显示出作为肠道健康和氧化应激调节的益生菌候选者的潜力.
这些菌株可以作为功能性食品成分或保健补充剂开发.
需要进一步的体内和临床研究来验证它们的治疗疗效.

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