保存的非编码序列CNS11是17型免疫细胞中Rorc转录的主要控制区域
Hao Zhang1,2, Dehui Chang1, Shiyuan Xie2,3
1Institute for Immunology and School of Basic Medical Sciences, Tsinghua University, Beijing, China.
Science advances
|February 25, 2026
概括
我们确定CNS11是控制Rorc基因表达的关键 cis调节元件. 这种元素对于表达RORγt的免疫细胞的发展至关重要,包括T辅助细胞17和先天性淋巴细胞.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 与网红酸受体相关的孤儿受体γt (RORγt) 对17型免疫细胞至关重要.
- 已知Rorc的跨调节机制,但 cis调节机制,特别是在先天性淋巴细胞中,理解程度较低.
研究的目的:
- 为了识别和描述控制Rorc表达的cis-regulatory元素.
- 阐明这些元素在RORγt表达免疫细胞的发育和功能中的作用.
主要方法:
- 通过CRISPR中介的删除已识别的cis调节元件 (CNS11).
- 在淘汰赛模型中分析免疫细胞发育和RORγt表达.
- 研究转录因子相互作用和调控循环.
主要成果:
- 鉴定出 CNS11 是 Rorc. 的一个必要的 cis 调节元件.
- CNS11对于维持T辅助细胞17中的RORγt表达至关重要.
- 中枢神经系统11删除取消了先天性淋巴细胞 (ILC3s),调节性T细胞和γδT细胞的发展.
- CNS11充当转录因子结合枢纽,与RORγt和其他辅助因子形成前循环.
结论:
- CNS11是Rorc转录在不同淋巴细胞群体中的主控制区域.
- 这一元素在先天性和适应性RORγt驱动免疫中起着不可或缺的作用.
- 了解CNS11的功能,可以了解免疫细胞的发育和潜在的治疗点.
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