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Updated: Feb 27, 2026

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Modeling Paracrine Noncanonical Wnt Signaling In Vitro
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平面细胞极性通过Wnt11的极化积累而出现
Yusuke Mii1,2,3,4,5,6, Minako Suzuki1,2,3,4, Hiroshi Koyama1,3
1National Institute for Basic Biology, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji-cho, Okazaki, Aichi 444-8787, Japan.
Science advances
|February 25, 2026
概括
Wnt11通过与核心PCP组件定位,独立于梯度来调节平面细胞极性 (PCP). 这就形成了一个相互放大循环,通过相互相互作用建立细胞极性.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 平面细胞极性 (PCP) 对于组织发育至关重要,由细胞内的不对称蛋白质定位建立.
- 已知Wnt蛋白质会影响PCP,以前认为梯度是主要的线索.
- 在PCP机构中Wnt信号的精确机制仍在调查中.
研究的目的:
- 为了研究Wnt11在Xenopus胚胎中的平面细胞极性 (PCP) 建立中的作用.
- 要确定Wnt11是否通过度梯度或其他机制来调节PCP.
- 为了阐明Wnt11和核心PCP组件在极性形成中的关系.
主要方法:
- 在PCP建立期间,在Xenopus神经板中对内源性Wnt11局部化的分析.
- 研究Wnt11极化对核心PCP组件的依赖性.
- 评估Wnt11对核心PCP组件两极分化的影响.
主要成果:
- 在Xenopus神经板中,Wnt11并没有沿PCP轴形成明显的梯度.
- Wnt11定位在具有核心PCP组件的细胞边界,表明细胞自主极化.
- Wnt11极化取决于核心PCP组件,反之亦然,这表明一个相互放大循环.
- Wnt11和核心PCP组件形成一个细胞间循环来协调极性方向.
结论:
- Wnt11以无梯度的方式调节PCP.
- 在Wnt11和核心PCP组件之间存在反循环,这对于确定细胞极性至关重要.
- Wnt11和核心PCP组件之间的局部,相互相互作用驱动PCP的形成.
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