通过门介导的双重LC-MS/MS同时量化血清中的脂肪和水溶性维生素
Weifeng Li1, Ka Luo2, Hou Qian1
1Department of Laboratory Medicine, Shenzhen Second People's Hospital/the First Affiliated Hospital of Shenzhen University, Shenzhen 518035, China.
Journal of pharmaceutical and biomedical analysis
|February 25, 2026
概括
本研究提出了一种双流液体色谱-并联质谱法 (LC-MS/MS) 方法,用于有效同时量化脂肪溶性维生素 (FSV) 和水溶性维生素 (WSV). 新的工作流在5分钟内将分析吞吐量翻一番,为复杂的维生素分析提供了实用解决方案.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 液体染色学-双重质谱法 (LC-MS/MS) 是维生素量化的标准.
- 同时测量脂溶性维生素 (FSV) 和水溶性维生素 (WSV) 由于它们的化学差异,在分析上很困难.
研究的目的:
- 开发一个务实的双流工作流,用于FSV和WSV的同时LC-MS/MS分析.
- 通过利用LC梯度的废弃细分来提高分析吞吐量.
- 验证方法的准确性和临床适用性.
主要方法:
- 实现了一种使用两个分阶段5分钟染色分离的双流工作流.
- 一个六端口转向被用来在质谱仪和废物之间交替换行列.
- 该方法通过将结果与使用患者血清的单一LC-MS/MS方法进行比较来验证.
主要成果:
- 双流方法有效地在5分钟的时间内将吞吐量翻一番.
- 观察到高精度,变化系数 (CVs) <10%的内部标准峰值区域.
- 方法比较表明与单一LC-MS/MS方法 (斯皮尔曼rho 0.950-0.997,低布兰德-阿尔特曼偏差) 有很强的一致性.
结论:
- 双流LC-MS/MS工作流提供FSV和WSV的高效和准确的同时量化.
- 这种方法提供了高吞吐量和临床一致性,使其可供实验室使用.
- 该方法利用现有的LC梯度浪费,优化分析时间和资源.
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