相关实验视频

Updated: Feb 28, 2026

Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies

Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies

Published on: October 6, 2022

由DNMT3A调节的中间前额叶皮质电路调节帕克利塔塞尔诱导的神经病痛

Lixia Tian¹, Rongrong Yao¹, Hui-Yuan Yi¹

¹Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, PR China; Institute of Neuroscience, Translational Medicine Institute, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, PR China.

European journal of pharmacology

|February 25, 2026

概括

在中部前额皮质 (mPFC) 中恢复DNA甲基转移酶3A (DNMT3A) 可以缓解神经病痛. 这突出了DNMT3A的特点.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
疼痛研究疼痛研究

背景情况:

中间前额叶皮层 (mPFC) 和它的连接参与了疼痛处理.
慢性疼痛机制,特别是神经回路重组,尚未完全理解.
帕克利塔塞尔 (PTX) 通过与DNA甲基转移酶3A (DNMT3A) 下调相关的mPFC低甲基化诱导神经病痛.

研究的目的:

研究DNMT3A调节的神经回路在帕克利塔塞尔诱导的神经病痛中的作用.
探索DNMT3A调节如何影响特定大脑电路中的疼痛过敏和基因表达.

主要方法:

在小鼠模型中利用了基于腺相关病毒的跨突触追踪策略.
在中部前额皮层 (mPFC) - 腹侧周水管灰色 (vlPAG) 和mPFC - 底侧杏仁体 (BLA) 电路中过度表达的DNMT3A.
在帕克利塔克塞尔诱导的疼痛条件下分析了mPFC中的基因表达特征.

主要成果:

在mPFC-vlPAG电路中DNMT3A的过度表达缓解了帕克利塔塞尔诱导的疼痛过敏.
在mPFC-BLA电路中的DNMT3A过度表达减少了热过敏症.
在DNMT3A调节后,确定了1131个与mPFC-vlPAG电路相关的差异表达基因.

结论:

中部前额叶皮层 (mPFC) DNMT3A在调节疼痛方面发挥着特定的作用.
mPFC-vlPAG和mPFC-BLA电路是通过mPFC DNMT3A发挥其疼痛调节效应的关键通路.
研究结果阐明了神经回路在应对慢性疼痛时的适应性重组.

关键词:

的DNA甲基转移酶3A.基本侧面的杏仁体中部前额叶皮层中部前额叶皮层神经病性疼痛是一种神经性疼痛.帕克利塔克塞尔 (paclitaxel) 是一种药物.腹侧侧周水管道灰色灰色

更多相关视频

The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice

The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice

Published on: July 16, 2014

Establishing a Mouse Model of a Pure Small Fiber Neuropathy with the Ultrapotent Agonist of Transient Receptor Potential Vanilloid Type 1

Establishing a Mouse Model of a Pure Small Fiber Neuropathy with the Ultrapotent Agonist of Transient Receptor Potential Vanilloid Type 1

Published on: February 13, 2018

相关概念视频

Analgesia and Pain Management

Analgesia and Pain Management

Pain is critical to various clinical pathologies, provoking an urgent need for effective management. Pain, whether acute or chronic, is a complex neurochemical process. Its alleviation depends on the type, with nonopioid analgesics effective for mild to moderate pain, such as musculoskeletal or inflammatory pain, while neuropathic pain responds best to anticonvulsants, tricyclic antidepressants, or serotonin/norepinephrine reuptake inhibitors. For severe acute or chronic pain, opioids may be...

Local Anesthetics: Differential Sensitivity of Nerve Fibers

Local Anesthetics: Differential Sensitivity of Nerve Fibers

Local anesthetics (LAs) block the sodium channels of nerve trunks, sensory nerve endings, and neuromuscular junctions. Although LAs can block all kinds of nerves, the sensitivity of nerve fibers differs according to nerve types and structures. LAs are known to block myelinated fibers faster than unmyelinated ones. Also, they block pain or sensory neurons at low concentrations without affecting the motor neurons involved in muscle contractions. This helps relieve labor pain without affecting the...

Nociception

Nociception

Nociception—the ability to feel pain—is essential for an organism’s survival and overall well-being. Noxious stimuli such as piercing pain from a sharp object, heat from an open flame, or contact with corrosive chemicals are first detected by sensory receptors, called nociceptors, located on nerve endings. Nociceptors express ion channels that convert noxious stimuli into electrical signals. When these signals reach the brain via sensory neurons, they are perceived as pain.