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应用下一代测序来调查新生儿重症监护病房中的Serratia marcescens疫情

Michela Bulfoni^1,2,3, Nicolò Gualandi⁴, Beatrice Krpan⁵

¹Department of Medicine, University of Udine, Udine, Italy. michela.bulfoni@uniud.it.

Antimicrobial resistance and infection control

|February 26, 2026

概括

下一代测序 (NGS) 在新生儿重症监护室 (NICU) 中发现了Serratia marcescens菌株之间的密切遗传联系,揭示了潜在的传播途径并告知了感染控制.

科学领域:

微生物学微生物学
基因组学就是基因组学.
传染病流行病学传染病流行病学

背景情况:

新生儿重症监护室 (NICU) 中的鼻腔感染对脆弱的婴儿构成重大风险.
塞拉蒂亚马尔塞森斯是一种关键的机会性病原体,有助于NICU相关的发病率和死亡率.
下一代测序 (NGS) 为微生物监测和疫情调查提供了先进的功能.

研究的目的:

开发和验证基于绘图的生物信息管道,用于对Serratia marcescens的菌株类型和植物遗传学分析.
调查S. marcescens从NICU环境中的患者和环境中分离出来的基因组相关性.
评估NGS在了解微生物传播动态和告知感染控制策略方面的实用性.

主要方法:

对18种来自患者的Serratia marcescens分离物和在新生儿重症监护室收集的环境抽样进行了回顾性分析.
使用Illumina MiSeq平台进行全基因组测序 (WGS).
开发和应用一个定制的,经过验证的生物信息学管道,用于基于单核酸多态 (SNP) 的基因分析.

主要成果:

全基因组测序 (WGS) 阐明了S. marcescens菌株之间的遗传学关系.
六个分离物体表现出高度的遗传相关性,表明潜在的克隆集群.
在患者样本 (血液,直肠拭子) 和环境来源的菌株中发现了相同的基因型,这表明了传播途径.

结论:

NGS为NICU感染和殖民的分子流行病学提供了高分辨率的见解.
基因组数据支持实时,基于证据的调整感染控制协议.
这种方法提高了患者的安全性,并有助于预防与医疗保健相关的疫情.

关键词:

进行健康监测.新生儿重症监护室 (NICU) 新生儿重症监护室下一代测序 (NGS) 是指下一代的测序.鼻腔感染感染鼻腔感染人类遗传学树 (Phylogenetic Tree) 是一个遗传学树.塞拉蒂亚 (Serratia marcescensens) 是一种的植物.全基因组测序 (WGS) 是一种全基因组测序.

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