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Overview Of Cell Separation And Isolation01:20

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells
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常规准备的细胞块的细胞性:来自国际研究的见解

Irena Srebotnik Kirbis1, Ivana Kholova2,3,4,5, Heini Huhtala6

  • 1Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

Cytopathology : official journal of the British Society for Clinical Cytology
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概括

细胞块制备方法在细胞性上显示出显著的变异性,影响诊断的准确性. 标准化这些方法对于可靠的细胞学样本处理至关重要.

关键词:
细胞块的细胞块是什么?细胞性是一种细胞性.细胞科学细胞学制备方法 制备方法

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科学领域:

  • 细胞病理学 细胞病理学
  • 组织病理学 组织病理学
  • 实验室医学 实验室医学

背景情况:

  • 固定在甲上,嵌入 (FFPE) 的细胞块 (CB) 是细胞学标本的标准.
  • 制备方法是可变的和非标准化的,往往导致低细胞性.
  • 低细胞性可能会损害细胞块的诊断效用.

研究的目的:

  • 为了评估例行准备的FFPE细胞块的细胞性.
  • 为了比较不同制备方法,实验室和样品类型的细胞性.
  • 识别细胞块制备中的变异性及其对诊断充分性的影响.

主要方法:

  • 一项多中心观察性研究评估了1817个FFPE细胞块.
  • 两个独立的审查者对H&E染色部分的细胞性进行了半定量评估.
  • 对准备方法,实验室和样本类型进行了差细胞细胞块 (非细胞或低细胞性) 的比较.

主要成果:

  • 在实验室中,缺乏细胞质的细胞块的比例差异很大 (4%至80%).
  • 差细胞细胞块的中位数比例在12% (Cellient) 到35% (Agar) 之间,没有统计学上显著的差异.
  • 在样品类型和制备方法之间和内部观察到细胞性的实质性变异.

结论:

  • 在不同的制备方法和实验室中,细胞质差的细胞块的比例存在显著的变化.
  • 显然需要改进细胞块充足性的处理和监测.
  • 细胞块制备的标准化对于确保诊断可靠性至关重要.