调查支气管支气管洗液细胞学与病原体识别和巩固深度在小牛的关系
Justine Clinquart1, Thomas Lowie1, Stan Jourquin1
1Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Salisburylaan 133, Merelbeke-Melle 9820, Belgium.
Journal of veterinary internal medicine
|February 26, 2026
概括
小牛呼吸道样本中中性粒细胞的百分比可能无法区分细菌和病毒性呼吸道感染. 然而,样本中的细胞内细菌可能表明机会性细菌感染.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 牛呼吸系统疾病 牛呼吸系统疾病
- 诊断细胞学 诊断细胞学
背景情况:
- 小体积,非支气管镜支气管洗 (nBAL) 是呼吸道感染 (RTI) 的小牛的常见气道采样技术.
- 对于鉴定细菌RTI的nBAL细胞学的诊断效用,特别是与疾病严重程度有关的诊断效用,仍然不清楚.
研究的目的:
- 调查特定病原体组 (病毒,机会性细菌[OB],Mycoplasmopsis bovis) 和nBAL液体中的细胞学发现之间的关联.
- 为了确定肺结合深度和细胞学参数之间的关系.
主要方法:
- 一项涉及87只在疫情期间出现RTI临床症状的小牛的横截面研究.
- 数据收集包括体检,胸部超声波,nBAL,细菌学,纳米孔测序和细胞学.
- 贝叶斯的等级模型被用来分析关联.
主要成果:
- 在nBAL液体中中性粒细胞的百分比与病原体群相关;病毒和M. bovis感染显示中性粒细胞的百分比较高,而机会性细菌感染显示出更大的变异性.
- 细胞内细菌 (≥1%的白细胞) 的存在显著增加了分离机会性细菌的几率 (OR,12.5).
- 更深的肺结合 (>3厘米) 与更高的中性粒细胞百分比和巨细胞的存在有关.
结论:
- 仅仅中性粒细胞的百分比不足以区分小牛的细菌和病毒RTI.
- 在nBAL样本中的细胞内细菌可以作为机会性细菌感染的有价值指标.
- 进一步研究以确定细胞内细菌的最佳值,可以提高诊断准确度.
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