从印度中部传播的登革热病毒血清型和基因型的分子特征
Manish Katiyar1,2, Kumar Sumit1, Ramaraju Ambati1
1Division of Virology and Zoonoses, ICMR-NIRTH, Jabalpur, Madhya Pradesh 482003, India.
Transactions of the Royal Society of Tropical Medicine and Hygiene
|February 26, 2026
概括
印度中邦的登革热病毒 (DENV) 分子表征显示,DENV-2宇宙系4占主导地位,其次是DENV-1基因型III. 这项研究为该地区流通的DENV菌株提供了关键的见解.
科学领域:
- 医学病毒学 医学病毒学
- 流行病学 流行病学
- 分子生物学分子生物学
背景情况:
- 登革热是一种主要的媒介性病毒性疾病,在热带和亚热带地区普遍存在.
- 了解登革热病毒 (DENV) 的分子流行病学对于有效的疾病控制至关重要.
研究的目的:
- 在印度Madhya Pradesh进行DENV血清型和基因型的综合分子表征.
- 为了确定在2023年期间在该地区流通的主要DENV菌株.
主要方法:
- 在2023年1月至12月期间,对2442例疑似DENV病例进行了回顾性分析.
- 使用非结构蛋白1 (NS1) 和IgM ELISA来确定血清流行率.
- 用PCR和部分包裹 (E) 基因序列的植物遗传学分析,在发烧5天内采集的样本上进行血清定型和基因定型.
主要成果:
- 在2442个疑似病例中,231个是DENV阳性 (9.45%).
- 通过PCR确认了16个DENV阳性样本,其中12例DENV-2和4例DENV-1病例.
- 遗传学分析发现DENV-2世界型基因型 (4系) 占主导地位,其次是DENV-1基因型III (GIII).
结论:
- 在2023年,DENV-2宇宙系4和DENV-1GIII是印度中部地区的主导菌株.
- 这些发现强调了该地区持续对登革热病毒进行分子监测的重要性.
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