唾液淋巴细胞表型与血液不同,并作为其他粘膜液的模型
Muruganantham Lillimary Eniya1, Shervin Dokht Sadeghi Nasab2, Albert Judith1
1The Voluntary Health Services, Chennai, India.
概括
流细胞计可以检测唾液中的各种免疫细胞,揭示出不同的粘膜免疫反应. 这种方法为研究影响粘膜免疫力的疾病提供了新的途径.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 口腔健康 口腔健康
背景情况:
- 口腔是身体粘膜免疫系统的关键部分,与众多抗原相互作用.
- 虽然粘膜免疫的可溶媒体得到了很好的研究,但免疫细胞表型仍然不太了解,这阻碍了疾病发病研究.
研究的目的:
- 审查免疫表型在粘膜分泌物的重要性.
- 证明流式细胞计对在刺激全口液 (SWMF) 中量化多种淋巴细胞表型的有用性.
主要方法:
- 进行了对免疫表型化技术的审查.
- 流细胞测量用于检测和量化来自119名参与者的SWMF和外围血液单核细胞 (PBMC) 中的淋巴细胞表型 (Th17,Th22,Tfh,Tregs,NKT,NK,ILC1,ILC2,ILC3).
主要成果:
- 流细胞计可靠地检测到SWMF中的各种免疫细胞表型.
- 在SWMF中,Th17,Th22,Tfh和NK细胞的频率高于PBMC中.
- 现型相关性表明,在SWMF中检测到的细胞具有特定的粘膜来源.
结论:
- 流细胞计对于检测SWMF中广泛的淋巴细胞表型是有效的.
- 这种技术对研究影响粘膜部位的疾病的免疫病原发生有前途.
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