相关实验视频

Updated: Feb 27, 2026

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Published on: February 2, 2016

Fgf8/18 在语言腹腔-背部模式中对抗Shh表达

Shuhui Yang¹, Junyuan Xue¹, Han Liu^1,2

¹Department of Oral Pathology, School of Stomatology, Dalian Medical University, Dalian, China.

Frontiers in cell and developmental biology

|February 26, 2026

概括

研究人员确定了Sonic hedgehog (Shh) 和纤维细胞生长因子8/18 (Fgf8/18) 作为舌头发育中的关键基因. 腹部Fgf8/18抑制背部Shh,建立了舌头的背部腹部轴.

科学领域:

发展生物学发展生物学
分子遗传学分子遗传学
头骨面部的发展

背景情况:

舌头发育机制,特别是背腔腹腔模式,仍然不太了解.
了解这种不对称性对于解释先天性舌头形至关重要.

研究的目的:

阐明了舌头背腔腹腔图案背后的分子机制.
为了确定关键的信号通路和基因参与建立语言不对称性.

主要方法:

利用转基因小鼠模型 (Osr2-cre KI;Rosa26R-Fgf8和Shh-cre;Rosa26R-Fgf8) 来研究基因表达.
进行了组织学测定和基因表达分析 (DEGs),以比较野生型和突变型的舌头.
研究了外源性FGF8/18和SHH补充对野生类型舌头基因表达的影响.

主要成果:

背部延伸Fgf8通过抑制背部介质细胞增殖和分化,破坏了背部-腹部不对称性.
声波刺 (Shh) 和相关基因在胚胎背舌上被激活,而与Fgf8/18相关的Lhx6在腹部表达.
腹部Fgf8/18抑制了Shh的背部延伸,表明在建立背部 - 腹部轴上的关键作用.

结论:

证实了舌头发育过程中背腔腹腔模式的存在.
确定了Shh和Fgf8/18作为语言背腹轴的关键调节器.
提供了对先天性语言形的分子洞察力.

关键词:

Fgf18 Fgf18 在线阅读 Fgf8 在线阅读背部腹部的模式模式.语言形语言形声波刺是一种刺.舌头的发育语言的发展.

更多相关视频

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

Published on: October 1, 2007

相关概念视频

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...